14/07/2026

Rethinking Protein in Pet Nutrition: From Traditional Sources to Alternative and Functional Proteins

In recent years, however, the discussion around protein has expanded significantly. Terms such as 'alternative proteins', 'novel proteins', and 'functional proteins' are increasingly used across the food and pet food industries. Much of this interest is driven by sustainability concerns and projections that global demand for protein will increase substantially as the human population approaches 10 billion by 2050 (FAO, 2022; OECD-FAO, 2023).
  This raises an important question: do we truly need entirely new protein sources to feed our pets, or should we focus on improving how we utilize the protein sources we already have?
  The answer likely lies somewhere in between. Traditional protein sources remain nutritionally robust, while emerging technologies and alternative ingredients offer opportunities to improve sustainability, functionality, and health outcomes—including effects on the gut microbiome.
Global Protein Demand and the Sustainability Debate   Projections toward 2050 suggest that global protein demand will rise sharply as the world population approaches 10 billion, with food demand expected to increase by about 60% (FAO 2018; FAO 2022; Henchion et al., 2017). At the same time, the global pet population continues to expand, with an estimated 900 million dogs and cats worldwide. Approximately 60% of these animals are concentrated in Europe, the United States, China, and Brazil.
  These trends have raised concerns about whether traditional livestock production alone can meet future protein needs. Critics often argue that feeding animal-derived proteins to pets competes with the human food supply and contributes to environmental pressures, such as land use, greenhouse gas emissions, and water consumption.
  However, the picture is more nuanced. A significant portion of animal-derived ingredients used in pet foods originates from rendered co-products of the human food system, including organ meats, trimmings, and fish processing residues. These ingredients represent an efficient form of nutrient recycling within the food supply chain (Boland et al., 2013).
  Advances in food processing technologies are also improving nutrient utilization, enabling greater recovery of proteins, peptides, and bioactive compounds from raw materials and by-products (Yuan et al., 2025). Improving how proteins are processed and utilized may therefore be as important as identifying entirely new protein sources.   Protein Requirements in Dogs and Cats   Protein requirements in companion animals are well established through decades of nutritional research. According to AAFCO guidelines (AAFCO 2026), the minimum protein requirement for adult dogs is approximately 18% on a dry matter basis, while growing puppies require 22.5%. Cats, as obligate carnivores, require higher protein intake, with minimum levels of 26% dry matter.
  In commercial diets, however, protein levels are typically higher. Many dry dog foods contain 22–32% protein, while cat foods often range from 30–40% protein. These levels reflect both nutritional needs and consumer expectations, as protein content has increasingly become associated with perceived diet quality.   Traditional Protein Sources: Still the Foundation   Animal-derived proteins remain central to pet nutrition because of their balanced amino acid profiles and high digestibility. Common ingredients include chicken meals, beef and lamb meals, fish meals, fish protein concentrations, and meat or organ by-products. These ingredients supply essential amino acids, such as lysine, methionine, and taurine and typically exhibit digestibility values around 85–90%, although digestibility can vary depending on the specific ingredient and processing conditions.
  Plant proteins also play a significant role in modern formulations. Ingredients such as soybean meals, pea protein, lentils, and chickpeas contribute valuable amino acids while offering flexibility and cost efficiency in formulation.
  Recent innovations in food processing have further improved plant protein functionality. Technologies such as fermentation, enzymatic hydrolysis, and advanced milling can increase digestibility while reducing anti-nutritional factors such as lectins and protease inhibitors (Yuan et al., 2025).
  These developments highlight an important point: innovation does not always require entirely new ingredients—sometimes it involves improving how existing ingredients are processed and utilized.   The Rise of Fresh and Minimally Processed Meat Ingredients   Over the past decade, the use of fresh meat or poultry has expanded significantly in dry pet food formulations. Many premium kibble products now incorporate fresh chicken, beef, or fish as part of their protein systems.
  In many cases, these fresh ingredients originate from mechanically deboned meat (MDM) or automated meat recovery (AMR) processes, which efficiently recover edible muscle tissue from carcasses after primary cuts have been removed. These ingredients provide highly digestible protein while improving palatability and consumer perception.
  Marketing narratives around 'fresh' ingredients have also been reinforced by the rapid growth of fresh and lightly cooked pet food formats. The fresh pet food market in the United States alone is estimated to exceed USD 3 billion and continues to grow (Packaged Facts, 2023). This trend illustrates how consumer expectations increasingly influence ingredient selection and processing strategies in the pet food industry.   The Rise of Alternative Proteins   While traditional proteins remain dominant, several emerging protein technologies are gaining attention.
  Insect Proteins
Insects, such as black soldier fly larvae, mealworms, and crickets are being explored as sustainable protein sources. Insect meals typically contain 40–65% protein and can be produced using relatively small amounts of land and water compared with conventional livestock production (van Huis, 2021).
  Insect proteins may contain bioactive compounds such as antimicrobial peptides and chitin that could influence immune function and gut health (Gasco et al., 2020). However, scaling production and achieving widespread consumer acceptance remain challenges.   Fermentation-Derived Proteins
Fermentation technologies represent another promising pathway. Microbial fermentation can produce single-cell proteins (SCP) using yeast, bacteria, fungi, or microalgae grown on various substrates (Matassa et al., 2016). These proteins can provide favorable amino acid profiles while requiring relatively small land footprints.
  Traditional fermentation methods—such as koji fermentation using Aspergillus oryzae—can also transform plant substrates into more digestible and nutritionally enhanced ingredients (Yuan et al., 2025). Advances in biotechnology now allow engineered microbes to produce specific peptides or proteins with targeted functional properties.   Cell-Cultured Proteins
Cellular agriculture represents one of the most technologically ambitious approaches to protein production. By culturing animal cells in controlled environments, it may eventually be possible to produce meat without traditional livestock production (Post, 2012). Although promising, this technology is still in its early stages—particularly for pet food applications—and faces challenges related to cost, energy use, and regulatory frameworks.   Protein and the Gut Microbiome
One of the most exciting areas linking protein nutrition and health is the gut microbiome.
  The gut microbiota plays a central role in digestion, immune function, and metabolic health in companion animals. Diet composition—including protein source and digestibility—can significantly influence microbial communities in the gastrointestinal tract (Handl et al., 2013).
  Highly digestible proteins reduce the amount of undigested nitrogen reaching the colon, minimizing the production of undesirable fermentation by-products, such as ammonia or biogenic amines.
  Conversely, certain peptides and amino acids may serve as substrates for beneficial microbial populations, contributing to the production of short-chain fatty acids and other metabolites that support intestinal health (Sandri et al., 2017).   Key Takeaways   The conversation around alternative proteins often frames the issue as a replacement of traditional proteins. Actually, the future of protein in pet nutrition will likely be more integrated and from this discussion, several key insights emerge:
  Traditional protein sources remain nutritionally efficient. Animal and plant proteins continue to provide reliable amino acid nutrition and will remain foundational ingredients in pet food.
  Alternative proteins expand the toolbox; insects, microbial fermentation, and cellular agriculture may complement existing protein systems.
  Processing technology and advances in fermentation, enzymatic modification, and ingredient recovery can improve nutrient utilization, reduce anti-nutritional factors, and enhance the functional value of protein ingredients.
  Consumer expectations are reshaping protein systems. The growth of fresh, minimally processed, and high-protein pet foods illustrates how market trends increasingly influence ingredient selection and processing technologies.
  Ultimately, the goal should not be to replace traditional proteins with alternatives but to develop a diverse and efficient protein ecosystem that supports sustainability, nutrition, and pet health. The key question is not only which proteins we choose, but how intelligently we use them. By Juan Gómez-Basauri, Ph.D. - MAGELLAN LLC
Source: All Pet Food Magazine   References
AAFCO 2026. Association of America Feed Control Officials. Official Publication Boland, M., Rae, A., Vereijken, J., Meuwissen, M. P. M., Fischer, A. R. H., van Boekel, M. A. J. S., Rutherfurd, S. M., Gruppen, H., Moughan, P. J., & Hendriks, W. H. (2013). The future supply of animal-derived protein for human consumption. Trends in Food Science and Technology, 29(1), 62-73.https://doi.org/10.1016/j.tifs.2012.07.002 FAO. 2018. The future of food and agriculture – Alternative pathways to 2050. Summary version. Rome. 60 pp. Licence: CC BY-NC-SA 3.0 IGO.https://openknowledge.fao.org/handle/20.500.14283/i8429en FAO. 2022. The future of food and agriculture – Drivers and triggers for transformation. The Future of Food and Agriculture, no. 3. Rome.https://doi.org/10.4060/cc0959en Gasco, L., Gabriele Acuti, Paolo Bani, Antonella Dalle Zotte, Pier Paolo Danieli, Anna De Angelis, Riccardo Fortina, Rosaria Marino, Giuliana Parisi, Giovanni Piccolo, Luciano Pinotti, Aldo Prandini, Achille Schiavone, Genciana Terova, Francesca Tulli & Alessandra Roncarati (2020) Insect and fish by-products as sustainable alternatives to conventional animal proteins in animal nutrition, Italian Journal of Animal Science, 19:1, 360-372.https://www.tandfonline.com/doi/pdf/10.1080/1828051x.2020.1743209 Handl S., German AJ, Holden SL, Dowd SE, Steiner JM, Heilmann RM, Grant RW, Swanson KS, Suchodolski JS. Faecal microbiota in lean and obese dogs. FEMS Microbiol Ecol. 2013 May;84(2):332-43. Epub 2013 Jan 24. PMID: 23301868.https://doi.org/10.1111/1574-6941.12067 Henchion, M. Hayes M, Mullen AM, Fenelon M, Tiwari B. Future Protein Supply and Demand: Strategies and Factors Influencing a Sustainable Equilibrium. Foods. 2017 Jul 20;6(7):53.https://pmc.ncbi.nlm.nih.gov/articles/PMC5532560/ Matassa, S., Boon N, Pikaar I, Verstraete W. Microbial protein: future sustainable food supply route with low environmental footprint. Microb Biotechnol. 2016 Sep;9(5):568-75. Epub 2016 Jul 8.https://pmc.ncbi.nlm.nih.gov/articles/PMC4993174/pdf/MBT2-9-568.pdf OECD/FAO 2023. OECD-FAO Agricultural Outlook 2023-2032, OECD Publishing, Paris.https://doi.org/10.1787/08801ab7-en. Post MJ. Cultured meat from stem cells: challenges and prospects. Meat Sci. 2012 Nov;92(3):297-301. doi: 10.1016/j.meatsci.2012.04.008. Epub 2012 Apr 11. PMID: 22543115.https://doi.org/10.1016/j.meatsci.2012.04.008 Sandri, M., Dal Monego S, Conte G, Sgorlon S, Stefanon B. Raw meat-based diet influences fecal microbiome and end products of fermentation in healthy dogs. BMC Vet Res. BMC Vet Res 2017; 13 (1):65.https://pmc.ncbi.nlm.nih.gov/articles/PMC5331737/ van Huis, A. Prospects of insects as food and feed. Org. Agr. 11, 301–308 (2021).https://doi.org/10.1007/s13165-020-00290-7 Yuan, Yi, Xinyao Wei, Yuhong Mao, Yuxue Zheng, Ni He, Yuan Guo, Ming Wu, Joseph Dumpler, Bing Li, Xu Chen, Xixi Cai, Jianping Wu, Yongqi Tian, Sihan Xie, Jeyamkondan Subbiah, Shaoyun Wang. Innovative Food Processing Technologies Promoting Efficient Utilization of Nutrients in Staple Food Crops, Engineering, Volume 50, 2025, Pages 229-244.https://doi.org/10.1016/j.eng.2025.04.014

08/07/2026

The Protein Matrix: Balancing Formulation, Operational Realities, and Consumer Demands

Today, as pets are increasingly humanized and elevated to the status of family members, the scrutiny placed on the ingredients in their bowls has never been higher. This is why, for this edition, I'm taking a different approach from my past articles and decided to write about the most scrutinized, expensive, and dynamic component of any pet food formula: protein.

The true value of a raw material is no longer dictated solely by its price per ton. Instead, it is defined by a complex matrix of nutritional efficacy, marketing appeal, and manufacturing viability. The universe of ingredients is expanding rapidly, introducing alternative proteins, functional extracts, and novel additives. However, to truly understand how these ingredients deliver value, we must examine how the menu of ingredients, including the different protein sources, complement each other in three dimensions: nutrition, consumer trends, and the realities of managing these materials on the factory floor.
Nutrition: How Proteins Complement Each Other
From a purely nutritional standpoint, a pet does not require specific ingredients; it requires specific nutrients. The goal of any formulator is to deliver a complete and balanced amino acid profile, alongside high digestibility and bioavailability, and palatability—because if the pet does not eat the product, all the effort has been done for nothing. Achieving this is rarely accomplished efficiently with a single protein source. Instead, formulation is all about complements.

Traditional animal protein sources, including chicken, beef, fish, and lamb meal, have long been the workhorses of the industry, offering high protein density and excellent palatability. However, they are often complemented with each other and with plant-based options, such as peas, soybeans, potatoes, or corn gluten meal.

Why blend them?  Because what one ingredient lacks, another provides. For instance, plant-based proteins can be highly digestible and lower the overall ash content of the formula, but they may be limited in essential amino acids (methionine or taurine precursors). By strategically pairing it with a marine protein source, such as salmon meal or a functional extract like yeast, a formulator can bridge the amino acid gap while maintaining a specific price point and functional target.

Furthermore, the rise of alternative proteins, including insects, single-cell proteins, and cultivated meats, is altering the formulation landscape. These ingredients are not just novelties; they can offer functional benefits, such as hypoallergenic properties or high levels of antimicrobial peptides. They 'play' within a formula, not just as bulk protein, but as functional additives that elevate the nutritional profile and the true value of the end product.
The Marketing Push: Premiumization and the Clean Label
While formulators focus on amino acids, digestibility, bioavailability, and palatability, the focus of marketing departments and consumers is the ingredient list and the product claims. In today's premium and super-premium segments, communication trends heavily influence product design, sometimes at odds with traditional formulation logic.

The push for 'clean label' products and limited ingredient diets is a prime example.  Higher-value segments are demanding shorter ingredient lists, driven by a consumer perception that fewer ingredients equate to a more natural, wholesome, and transparent product. This creates a significant challenge: how do you deliver a perfectly balanced amino acid profile when your marketing brief restricts you to a single protein source and a single carbohydrate source?

This restriction has driven the industry toward novel proteins. These new ingredients, such as rabbit, kangaroo, wild boar, herring, venison, and duck, are commanding premium prices—they serve a dual purpose. First, they are highly effective for pets with suspected food sensitivity or allergies to common proteins like poultry or beef. Second, they provide a powerful narrative for marketing and communication. Novel proteins instantly differentiate a brand on a crowded retail shelf, communicating exclusivity and premium quality.

However, the true value here also hinges on the traceability of origin. Consumers put value into knowing that the rabbit was sustainably sourced or if the salmon was wild. Traceability has evolved from a supply chain buzzword into a non-negotiable consumer demand and a core pillar of a brand's value proposition. The story behind the ingredient is now just as important as the ingredient itself.
The Factory Floor Reality: Navigating Production Pain Points
A recipe can look perfect on paper and test brilliantly in a consumer focus group, but it must be feasible to manage within an existing manufacturing process. Managing diverse and novel proteins in a high-volume factory introduces pain points that question the concept of "true value."

One of the most persistent challenges is managing the natural variability of raw materials, particularly animal by-product meals. Meat and bone meals, for instance, can vary significantly from batch to batch depending on the rendering process and the exact source materials. A classic factory pain point is color variation. If a specific batch of chicken meal contains a higher concentration of blood, the resulting kibble will be noticeably darker. While the product remains entirely safe and nutritionally sound, this visual inconsistency can inevitably lead to consumer complaints. Pet owners are conditioned to expect absolute uniformity, and a dark batch of kibble is often misinterpreted as burnt or spoiled. Managing this requires strict supplier specifications, advanced mixing techniques, and sometimes, the reluctant use of colorants to standardize the final appearance.

Beyond color, different proteins behave differently during extrusion. Plant proteins often require different specific mechanical energy and moisture inputs compared to animal proteins. High levels of fresh meat, while highly appealing on an ingredient label, introduce massive amounts of water into the formula, which must be managed to ensure the kibble expands properly and dries to a safe moisture level to prevent mold.
Conclusion: Redefining True Value
The universe of pet food ingredients is undoubtedly expanding, but 'true value' is not found simply by adding the newest, trendiest protein to a formula. True value is achieved at the intersection of three distinct disciplines.

It requires the formulator's skill to blend complementary amino acid profiles for optimal animal health. It demands the marketer's insight to select ingredients that resonate with consumer demands for transparency, limited ingredients, and sustainable origins. And crucially, it relies on the factory expertise to handle the physical realities, variabilities, and organoleptic challenges of processing raw materials at scale.

As the industry continues to innovate beyond price, the brands that succeed will be those that master this complex matrix, ensuring that every raw material earns its place in the bowl: nutritionally, commercially, and operationally. By Felipe Martinez R.
Source: All Peto Food Magazine

By Felipe Martínez R.

12/06/2026

 Leveraging Artificial Intelligence to Predict Pet Food Palatability

WHY THIS MATTERS
Palatability is one of the strongest drivers of product success in pet food. Yet, predicting it remains challenging. Development still relies heavily on iterative animal trials, which are time-consuming, costly, and often identify failures late in the process.
  At AFB, we are changing this approach by using artificial intelligence (AI) to predict palatability outcomes before testing. By combining historical data with formulation knowledge, we help transform development from trial-and-error into a more targeted and efficient process.
  THE CHALLENGE
Palatability is the result of multiple interacting factors, including ingredients, inclusion levels, fat systems, processing, and animal variability. These interactions are complex and non-linear, making outcomes difficult to anticipate.
  Because of this complexity, traditional approaches struggle to efficiently explore formulation space and consistently deliver high-performing solution.   A NEW APPROACH: FROM TESTING TO PREDICTING
Instead of relying only on experiments, we integrate data across trials and formulations to identify patterns and predict outcomes.
  Our models transform inputs such as recipe composition, ingredient levels, and processing conditions into clear guidance: which solutions are most likely to succeed and which carry higher risk.
    FROM DATA TO DECISIONS
Our approach converts complex datasets into practical insights that guide development. Rather than focusing only on performance, we also consider how reliable each result is.
  By combining performance with confidence, we generate a clear prioritization of what to test next. This allows teams to focus on the most promising formulations and avoid unnecessary trials.
    WHAT THIS ENABLES

This predictive approach allows us to:
  Identify high-potential solutions earlier Reduce experimental burden Focus on the most impactful ingredients Improve consistency of results
  In practice, large experimental spaces can be narrowed down to a smaller set of high-confidence candidates, accelerating development and improving outcomes.   A SMARTER WAY TO DEVELOP PALATANTS
Artificial intelligence does not replace expertise—it enhances it. Scientific knowledge remains central to interpretation and validation, while predictive models help guide decisions and reduce uncertainty.
  By combining data, science, and AI, we enable a more efficient and confident approach to palatability development. Source: AFB International

26/05/2026

Colmax: Natural Choline for Neural, Metabolic, and Digestive Well-being in Pets

In companion animal nutrition, well-being begins long before food reaches the bowl. Ingredient quality and nutrient balance are key factors in sustaining the vitality and metabolic health of dogs and cats.

Among these nutrients, choline plays a fundamental role. Its presence in the diet contributes to proper liver function, lipid transport, and the development of the nervous system. Dogs and cats do not synthesize sufficient amounts on their own, making the inclusion of this ingredient essential.

Colmax is a natural source of choline and inositol developed by Adinnova to help regulate liver, lipid, and energy metabolism. Its plant-based formulation enables this essential nutrient to be incorporated into different pet food formulations in a stable and safe manner.  
Why Is Choline Key in Pet Nutrition?
Choline is involved in fundamental physiological processes, particularly liver metabolism, fat transport, and nervous system function. When dietary intake is insufficient, liver disorders, reduced vitality, or muscle weakness may occur.

Traditionally, the most widely used source of choline in animal nutrition has been choline chloride. However, this molecule presents certain technological limitations. It is a hygroscopic and reactive compound that can interact with other dietary ingredients, affecting the stability of sensitive nutrients during feed processing and storage. Among the most common effects are the oxidation of vitamins, pigments, and amino acids. Moreover, its origin is associated with petrochemical byproducts.

Natural sources of choline represent an alternative aimed at overcoming these limitations. Plant-based choline is associated with phospholipids such as phosphatidylcholine, which are in the cellular membrane structure and contribute to its biological stability.
Adinnova's Natural Choline
Adinnova's source of choline and inositol, Colmax, was developed to contribute to metabolic balance in animal nutrition. Its formulation combines plant-based choline with functional compounds that support cellular performance.

Its components include phospholipids, such as phosphatidylcholine and phosphatidylinositol, as well as coadjuvants, for example, butyric acid and plant extracts, that support intestinal and liver health and nutrient absorption. This combination helps optimize lipid transport and utilization, helping metabolic balance and pet vitality through nutrition.

Another relevant characteristic is its technological stability. Colmax is a fluid, non-hygroscopic presentation that withstands the thermal treatments commonly used in pet food processing, as these molecules remain active and maintain absorption capacity. In turn, its use allows lower inclusion levels in the formula compared with synthetic choline sources, optimizing formulation efficiency and economic performance.
The Impact of Colmax in Pet Care
Colmax provides choline, a pseudovitamin that dogs and cats do not synthesize in sufficient amounts. Its presence in the diet helps reinforce the integrity of cell membranes and participate in neurometabolic processes linked to vitality and normal body function.

This nutritional additive supports the healthy development of the brain, heart, liver, muscles, and nervous system, contributing to the overall well-being of animals through nutrition.

Its stable formulation also allows easy incorporation into pet food. The recommended dosage in pet care ranges from 150 to 500 g/ton of feed, and it can replace choline chloride (60%) at an approximate ratio of 1-4, optimizing the use of space in the diet.

By supporting cellular function, Colmax helps sustain the vitality and well-being of dogs and cats throughout all life stages. Its nutritional contribution reinforces cell membrane integrity, supports neuronal development, and contributes to pets' overall health.
Studies on Colmax
This biosolution has been supported by scientific evaluations aimed at understanding its effect on animal metabolism. During 2024 and 2025, Adinnova carried out studies in collaboration with the National Institute of Agricultural Technology (INTA) to analyze productive, metabolic, and physiological parameters, including performance, liver function, and tissue analysis. The results validated the contribution of Colmax as a nutritional additive that supports the general well-being of animals.  
Gene expression studies were also conducted using advanced sequencing technologies to observe how the organism responds to its inclusion in the diet. Analyses have shown that Colmax modulates several metabolic pathways linked to nutrient utilization and cellular energy production.

Lower activation of genes associated with cell proliferation processes was observed. When dysregulated, these mechanisms are often linked to different pathologies, including tumor processes such as cancer. In other words, Colmax supports an active and balanced metabolism without stimulating cellular mechanisms associated with uncontrolled proliferation. By Adinnova
Source: All Pet Food Magazine

About Adinnova
Adinnova is an Argentine company, present in international markets, dedicated to the development of natural additives for animal nutrition. Each biosolution integrates science and innovation applied to wellbeing and productivity.

More information on our website: adinnova.com.ar