Latest content from Ingredients

Baked Biscuits Utilizing Spray Dried Plasma Products
Other microingredients

2+ MIN

Baked Biscuits Utilizing Spray Dried Plasma Products

Background Information   Spray dried plasma (SDP) or Cravings (hydrolyzed plasma) are high-protein ingredients utilized in pet food for functional texture properties, enhancing palatability, or supporting overall health.   Study Objective   The study objective was to evaluate how SDP or Cravings inclusion impacts texture and palatability of baked biscuits utilizing wheat gluten.   Experimental Procedure   The study evaluated palatability and texture of baked biscuits utilizing 1% SDP or Cravings to replace wheat gluten in control formula. Palatability testing was conducted with 20 dogs for a 2-day test. Different ingredient sources and processing conditions were used in each study accounting for differences in texture of final product.
  Baked biscuits were made by preparing the dough utilizing a KitchenAid mixer equipped with a dough hook, rolling out by hand to a set depth and size, and then baking to moisture level of less than 10%. Three replicate batches of each formula were completed for analysis per study.
  Texture was measured on a TA.XT Plus utilizing an adjustable bridge with a rounded-end knife probe for a 3-point bend. Baked biscuits of 2 cm × 7 cm were placed over the two-bridge span spaced 2.5 cm apart to measure maximum force to break (hardness), fracturability, and stiffness to determine texture. Five baked biscuits per treatment were analyzed for texture from each replicate batch. Digital calipers were utilized to measure biscuit dimensions and volume.   Proof of Concept: Test Formula
    Results: Texture
 
Results: Palatability
 
Summary
SDP and Cravings can be incorporated into baked biscuits as a processing aide to impact texture depending on ingredient matrix and target, while SDP also improves palatability. Overall, both SDP and Cravings can be an alternative to vital wheat gluten to maintain or enhance product quality.
  By Joy Campbell and Angela Smith - APC
Source: All Pet Food Magazine

Impact of Insect Protein Meals on Pet Food Palatability
Palatants

7+ MIN

Impact of Insect Protein Meals on Pet Food Palatability

This article will focus on the incorporation of insect protein meals into pet food and the evaluation of its impact to palatability in dogs and cats' diets by analyzing the last public research available on this topic.   Insect Materials as Pet Food Ingredients
Last decade, the use of insect ingredients as balanced sources of proteins and fats has become a global reality. In each continent, we can find research centers and universities with projects focused on insect materials and their use in humans, livestock and pet food diets.
  Big scale production has also improved, so big hurdles to becoming profitable for producers and affordable for customers are about to be overcome. This positions insect meals and oils as relevant sources of proteins and lipids for the pet food industry.²
  Insect ingredients not only meet the nutritional requirements for pets but are also a great tool for claiming sustainability, lower carbon footprint and innovative ingredients, just to name a few.³
  While insect proteins can have a lower environmental footprint than most animal proteins, this is not as significant for the pet food industry, since pet foods mainly incorporate low environmental impact animal by-products from the rendering industry.⁴
  Currently, the main sources of insect oils and meals are black soldier fly, mealworm and cricket. They are considered as good alternative protein sources and mostly have adequate amino acid levels, meeting the standard requirements for dogs and cats.⁵
  In insect materials, protein is the nutrient composing the largest fraction, with values ranging from 40% to more than 60 % on a dry matter basis.
  Fat makes up the second largest fraction, and generally, insect materials contain lower levels of ash compared to other animal protein sources (see Table 1). Insects are also packed with micronutrients, including iron, zinc, calcium, and B-vitamins, which are vital for various bodily functions.⁶
  Table 1. Average nutrient composition of selected form of insects on a dry matter basis.   One key property of insect ingredients is that it is possible to modulate their nutritional composition by changing the feeding during the insect rearing. Several studies confirmed this by investigating the impact of different diets on the content and amino acid profile, lipids and other nutrients in insect protein meals and oils. Consequently, we could find insect meals and oils from the same species but with a different amino acid profile or fatty acid profile.¹² Therefore, insects represent versatile raw materials for the pet food industry with fast growing expectations in the market.
  As per Rabobank's research of 2021, see Figure 1, the global volume of insect ingredients incorporated in feed and pet food is expected to grow up to 500K MT by 2030, predicting pet food to be the second largest market.¹³
  Figure 1. Increase of the insect production and use in feed and pet food.   According to a review done in 2023, there are 43 insect-based pet food brands active around the globe, 35 of which are operating in Europe. Black soldier fly and mealworm are the most used species in pet foods.¹⁴
  Despite the various advantages of using insect ingredients there are other aspects that must be evaluated when applying them in pet food. This includes potential allergic reactions, contamination risks during production, stability and shelf-life concerns and the ability to meet the nutritional needs of cats and dogs in a specific recipe.   Insect Ingredients Effect on Palatability
Another important factor and key to the success of a diet is 'palatability'. Palatability is a critical factor in pet food acceptance. Cats are notoriously picky eaters, and their willingness to consume food is influenced by its taste, aroma, and texture. Therefore, understanding how insect protein affects these sensory attributes is essential.
  Insects have unique flavor profiles that can vary significantly between species. For instance, crickets have a nutty taste, while mealworms can be more neutral. These flavors can be both an advantage and a challenge when formulating palatable pet foods.
  Edible insects were first promoted as a sustainable method for food waste bioconversion, and it seems this practice is already implemented in Asia. However, how the heterogeneity of food waste sources affect fat oxidation, flavor and palatability of the final insect meal for pets is not well understood.
  To maintain or improve palatability, manufacturers use flavor enhancers, fats, and other ingredients to mask or complement the natural taste of insect protein. For example, adding chicken fat or liver flavor can make insect-based pet food more appealing.
  Currently there is limited reliable research on palatability of insects in pet foods, but we expect more long-term research to be done in the coming years to allow a standard and better evaluation of this factor. However, we could find in the literature some interesting palatability studies thanks to a review done by Bosch and Swanson.¹⁵
  Some studies found that regarding palatability of insects, dogs and cats showed different preferences depending on the insect species and the inclusion rate of the insect ingredients.
  In 2018, Beynen reported that dogs showed a preference for dry foods containing black soldier fly larvae meal over those with yellow mealworm meal (intake ratio of 60:40; n=10), whereas cats preferred the yellow mealworm-based food (40:60; n=10). Both insect meals represented 30 % of the total crude protein in these diets.¹⁶
  In 2020 Kilburn et al. showed that a banded cricket meal inclusion rate of 8, 16 or 24 % in extruded pet foods did not affect the intake in dogs (n=8).¹⁷
  Regarding cats' palatability, Paßlack and Zentek published in 2018 that 3 out of 10 cats had refused a food containing 35 % black soldier fly meal and 3 cats had an intake between 78 and 87 % of the food offered.¹⁸
  For a food containing 22 % black soldier fly meal, one cat vomited and then refused the food completely and two cats had lower food intakes (83 and 88%).¹⁷
  In cats fed a diet containing 5 or 20 % of black soldier fly meal for 2 days (n=20 per diet), 38 and 54 % of food was consumed. None of the cats rejected the 5% black soldier fly diet, whereas one cat rejected the 20 % black soldier fly meal diet.   Future Prospects of Insect Protein in Pet Nutrition
Insect protein represents a promising frontier in pet nutrition, offering a nutritious alternative to traditional proteins. The impact of insect protein on pet food palatability is a critical factor that manufacturers are actively addressing through research, innovation, and consumer feedback.
  While insect protein holds great promise, there are challenges that need to be addressed to ensure its widespread adoption in the pet food industry.
By Cristina Murcia García, PhD, Technical Service Manager | Kemin Nutrisurance EMEA
Source: Kemin Pet Food & Rendering

About the author
Dr. Cristina Murcia García gained her PhD in Chemistry with focus on red-ox reactions from the University of Bonn (Germany). Since then, she has specialized in managing oxidation in rendering and pet food products. She works as Technical Service Manager for Kemin Nutrisurance Europe where she provides solutions to improve raw materials and pet food safety and shelf-life.
References
1 World Resources Institute 2 FAO. 2022. Is the time ripe for using insect meal in aquafeeds? Bangkok 3 FEDIAF Fact sheet 2024. Insect-based ingredients in pet food 4 EFPRA – White Paper, Life Cycle Assessment of Rendered Products 5 Van Huis, A., Van Itterbeeck, J., Klunder, H., et al. 2013. Edible Insects: Future Prospects for Food and Feed Security. Rome: Food and Agriculture Organization of the United Nations (FAO) 6 Rumpold, B.A., Schluter, O.K. 2013.Nutritional composition and safety aspects of edible insects. Mol. Nutr. Food Res. 57, 802–823; and *Kemin Internal Documents 7 Makkar, H. et al. 2014. State-of-the-art on use of insects as animal feed. Anim. Feed Sci. Technol. 197, 1–33 8 Rumpold, B.A., Schluter, O.K. 2013. Nutritional composition and safety aspects of edible insects. Mol. Nutr. Food Res.  57, 802–823 9 Ramos-Elorduy, J., Pino Moreno, J.M., Correa, S.C.1998. Edible insects of the state of Mexico and determination of their nutritive values. Zoologia, 69, 65–104. 10 Józefiak, D., Józefiak, A., Kiero ´nczyk, B., Rawski, M., Swi ˛atkiewicz, S., Długosz, J., Engberg, R.M. 2016. Insects—A natural nutrient ´ source for poultry—A review. Ann. Anim. Sci. 16, 297–313. 11 Kemin Internal documents 12 Biancarosa, I., Araujo, P., Biemans, D., Bruckner, C.G., Waagbø, R., Torstensen, B.E., Lock, E.J. 2017. Modulation of nutrient composition of black soldier fly (Hermetia illucens) larvae by feeding seaweed-enriched media. PLoS One.  Aug 24;12(8) 13 Rabobank research, 2021 14 Journal of Asia-Pacific Entomology, ISSN: 1226-8615, Vol: 26, Issue: 1, Page: 102020, 2023 15 Bosch, G., Swanson, K. S. 2021. Journal of Insects as Food and Feed, 7(5): 795-805 16 Beynen, A.C., 2018. Insect-based petfood. Creature Companion: 40-41 17 Kilburn, L.R., Carlson, A.T., Lewis, E. and Rossoni Serao, M.C., 2020. Cricket (Gryllodes sigillatus) meal fed to healthy adult dogs does not affect general health and minimally impacts apparent total tract digestibility. Journal of Animal Science 98: 1-8. 18 Paßlack, N. and Zentek, J., 2018. Akzeptanz, Verträglichkeit und scheinbare Nährstoffverdaulichkeit von Alleinfuttermitteln auf Basis von Hermetia-illucens-Larvenmehl bei Katzen. Tierärztliche Praxis Kleintiere 46: 213-221

Beyond Dry Food: Functional Additives in the Pet Food Market
Other microingredients

5+ MIN

Beyond Dry Food: Functional Additives in the Pet Food Market

By Erika Stasieniuk

The answer is in the intersection between nutritional science, consumer behavior, and market strategy.    What Do Pet Owners Value?   The increase in pet humanization has transformed pet owners' view about food. Nowadays, they not only look for satiety but also for disease prevention, healthcare, and well-being promotion, which reflects the concern about their own nutrition and health. 
  This behavior shift is confirmed by Hobbs Jr. and Anderson (2024), who studied which healthy properties claims add value to consumers. Pet owners are willing to pay more for certain benefits, according to the authors of a study on nearly 1,600 dry dog foods.
  The statement 'allergy relief' showed a surcharge of up to 22.7%. 'Digestive health' and 'sensible skin' also correlate with more perceived value. Generic terms such as 'vitamins and minerals' or 'dental care' were associated with a lower willingness to pay, indicating that they are perceived as basic attributes rather than differentiators. This data offers a strategic orientation for the industry—clear and specific claims with visible benefits are more valuable and explain premium positioning.   Functional Additives Applications in Canine and Feline Food   Functional statements that go beyond marketing are backed up by proven efficacy ingredients.
  Additives have always been an integral part of formulations from technological and sensorial goals to nutritional and zootechnical purposes. However, its function has shifted: in fact, it has become a strategic tool for differentiation and commercial value.
  Prebiotics, natural antioxidants, essential fatty acids, and others were increasingly added to formulations both for their technical functionality and their commercial appeal. Among them, ingredients with functional effects stand out in gut health, e.g., mannan oligosaccharides (MOS), fructo-oligosaccharides (FOS), and beet pulp.
  According to Singla and Chakkaravarthi (2017), prebiotics such as inulin and FOS are non-digestible fibers serving as substrates for beneficial gut bacteria (Bifidobacterium and Lactobacillus). Classic studies highlight their benefits:
  Balance in gut microbiota (Gibson y Roberfroid, 1995). Better mineral absorption, such as calcium and magnesium (Scholz-Ahrens et al., 2007). Immune response modulation (Lomax and Calder, 2009). Reduction of inflammatory compounds in the colon (Slavin, 2013).
  MOSs are functional carbohydrates extracted from yeast cell walls, mainly from Saccharomyces cerevisiae. These compounds serve as allies in gut health as they prevent pathogenic bacteria from adhering to the mucosa. MOS are in the binding site of epithelial cells to prevent the adhesion of harmful microorganisms, a mechanism known as competitive exclusion. In addition to the protective effect, MOSs stimulate defense cells (macrophages) by exceeding mannose receptors in glycoproteins of the cell surface (Macari and Maiorka, 2000; Strickling et al., 2000).
  Beet pulp is a fermented fiber, rich in soluble and insoluble fiber. It contributes to the balance of gut microbiota, enhances fecal consistency, and improves bowel transit. Moreover, its partial fermentation in the colon generates short-chain fatty acids (SCFAs), such as butyrate, which serve as an energy source for colonocytes and contribute to the intestinal mucosa integrity (Swanson et al., 2002).
  The statements 'enhance digestive health' or 'balance of intestinal flora' are credible when included in ingredient formulations, such as inulin, FOS, MOS, and beet pulp. The connection between functionality and labeling is essential to building confidence and perception of real value.   From the Ingredient to the Positioning in the Market   Brands face the challenge of identifying technical complexity in clear, attractive, and accessible messages. 
  Instead of mentioning 'inulin' in the list of ingredients, it is better: 'natural prebiotic fiber of chicory roots that promotes gut health.'
  This approach trains the consumer and strengthens the legitimacy of the functional statement. Functional ingredients not only must be listed but also must be perceived and valued as differentiators.
  Other relevant examples:
  Omega-3 (fish oil and linseed): Related to dermis health, coat shine, and joint well-being. Vitamin E and selenium: Natural antioxidants that strengthen the immune system. Glucosamine and chondroitin: Usually present in food for senior dogs or big breeds, which support their beneficial effect in joints.   The Pillars of their Commercial Efficacy   Science-based functional ingredients with an effective dose proven by studies. Clarity and transparency in the communication of their benefits. Alignment between their value proposal and price positioning.   Opportunities in a New Scenario   The search for healthier, specific, and functional food is no longer a trend—it is the standard in the pet food market.
  In an increasingly competitive market, brands successfully combining science, formulation, and an effective communication strategy will be better positioned to address a more demanding and informed consumer.
  Prebiotics and other functional additives, when used with technical expertise and purpose, can make dry food a real tool for the health and well-being of dogs and cats. The market is open to brands that offer more than nutrition: those that build trust.
  Ideal labeling goes beyond attractive promises. It must convince consumers by presenting a science-aligned list of ingredients that support each statement. By bridging the gap between formulation and transparent communication, the industry evolves in promoting a longer and healthier life for pets.
  By Marcos Borges S. Rosa, Marcela Lobo N. Lima, and Erika Stasieniuk
Source: All Pet Food Magazine
  About the authors
Marcos Borges S. Rosa is a zootechnician with a postgraduate degree in canine and feline nutrition, and a master's in veterinary science from UFU (Federal University of Uberlândia). He offers in-person and online nutritional services for dogs and cats. Contact: www.marcosnutripet.com | Instagram: @marcosbsrr

Marcela Lobo N. Lima is a veterinarian with a postgraduate degree in canine and feline nutrition from Unyleya and works in formulation. Contact: marcela.nasc21@hotmail.com | Instagram: @marcelanasc

Erika Stasieniuk is a zootechnician with a doctorate in canine and feline nutrition from UFMG. She is the founder of SFA Consultoria and works as technical consultant in the development of pet food and ingredients. Contact: erika_stasieniuk@sfa-consultoria.com | Instagram: @erikastasieniuk
  References
Gibson, G. R., e Roberfroid, M. B. (1995). Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. The Journal of Nutrition, 125(6), 1401–1412.
Hobbs Jr., L., e Anderson, A. (2024). Assessing Price Premiums of Health and Wellness Product Attributes in Pet Food: Implications for Product Positioning and Marketing Strategies.
Lomax, A. R., e Calder, P. C. (2009). Prebiotics, immune function, infection and inflammation: a review of the evidence. British Journal of Nutrition, 101(5), 633–658.
Singla, V.; Chakkaravarthi, S. (2017). Applications of prebiotics in food industry: A review. Food Science and Technology International 23(8) 649–667. DOI: 10.1177/1082013217721769.
Macari, M.; Maiorka, A. Função gastrintestinal e seu impacto no rendimento avícola. In: CONFERÊNCIA APINCO'2000 DE CIÊNCIA E TECNOLOGIA AVÍCOLAS, 2000, Campinas.
Scholz-Ahrens, K. E., et al. (2007). Prebiotics, probiotics, and synbiotics affect mineral absorption, bone mineral content, and bone structure. The Journal of Nutrition, 137(3 Suppl 2), 838S–846S.
Slavin, J. (2013). Fiber and prebiotics: mechanisms and health benefits. Nutrients, 5(4), 1417–1435.
Swanson, K. S., et al. (2002). Fruit and vegetable fiber fermentation by gut microflora from canines. Journal of Animal Science, 80(10), 2725–2734.

Understanding the Role of Antioxidants in Pet Food Preservation
Other microingredients

3+ MIN

Understanding the Role of Antioxidants in Pet Food Preservation

What Are Antioxidants and Why They Matter
An antioxidant is a molecule that protects cells from damage caused by free radicals. Antioxidants in pet food prevent fats and oils from breaking down through oxidation. Oxidation can lead to rancidity, off-flavors, reduced aroma, and the loss of essential nutrients, ultimately affecting the pet's willingness to consume the food.
  Sara explains:
  'Without proper antioxidant protection, even a well-formulated product can lose its nutritional integrity and appeal over time. Antioxidants are not just additives—they're crucial for product performance.'
  For more details on how oxidation control in pet food works, see Kemin's Oxidation Control solutions.   The Bigger Picture: Why Antioxidants Impact Palatability
Oxidation doesn't just affect nutrients—it also influences flavor, aroma, and palatability, which are critical to pets' acceptance of food. Sara notes:   'Even minor changes in flavor or smell can lead pets to reject a product. Using antioxidants correctly ensures that the food not only remains nutritious but also appetizing for cats and dogs.'
  For more on enhancing flavor and pet acceptance, explore Kemin's Palatability Solutions.   Choosing the Right Antioxidants for Your Product
Not all antioxidants are created equal, and their effectiveness depends on the type of fat used, processing methods, and storage conditions. Sara highlights that selecting the right pet food antioxidants is about understanding these interactions:
  Type of fat or oil matters: Some fats oxidize faster than others, requiring different antioxidant strategies.
  Processing conditions affect stability: Heat, moisture, and extrusion processes can impact how antioxidants perform.
  Storage and packaging are critical: Packages are exposed to a variety of conditions over their lifecycle — from heat and humidity during transport to oxygen exposure once opened by the pet owner. That's why Kemin tests antioxidants under worst-case scenarios to ensure stability and performance.
  Kemin offers tailored solutions to match these needs, including PET-OX® synthetic antioxidants for overall stability, TERMOX® ethoxyquin-free antioxidants for sensitive formulations, and NATUROX® natural antioxidants for clean-label products.   Strategies for Effective Antioxidant Use
Sara shares practical tips for incorporating antioxidants in pet food:
  Evaluate raw materials carefully: Understand the oxidative potential of fats and oils used in the recipe.
  Match antioxidants to processing methods: Choose antioxidants that maintain stability through extrusion, baking, or other manufacturing processes.
  Monitor storage and handling: Use proper packaging, limit air exposure, and educate distributors on optimal storage practices.
  Test and validate: Regularly monitor oxidation levels and sensory attributes to ensure products meet quality expectations.
  By following these strategies, manufacturers can deliver consistent, safe, and palatable products that pets enjoy while maintaining nutritional integrity.
  Pet food antioxidants are more than just additives—they are essential tools for maintaining pet food quality, safety, and palatability. By carefully selecting antioxidants, considering processing conditions, and monitoring storage, manufacturers can ensure their products remain fresh, nutritious, and appealing to pets.
  For more in-depth insights, listen to the full podcast episode with Sara Cutler below. Source: Kemin Nutrisurance

Delayed Dry Palatant Coating: Does It Affect Palatant Recovery Rate and Palatability?
Palatants

3+ MIN

Delayed Dry Palatant Coating: Does It Affect Palatant Recovery Rate and Palatability?

As we mentioned, in dry pet food manufacturing, the standard palatant application sequence typically follows three steps: (i) fat spraying, (ii) liquid palatant spraying, (iii) dry palatant application. Most fat must be heated (30-40 °C) to remain liquid. Once the fat is applied, palatants are usually layered on top, as depicted in Figure 1.
  However, in certain situations—such as when the dry palatant is applied at a different location—it may be necessary to coat pre-fat-coated kibbles with dry palatants at a later stage.
  This study investigates two key questions:
  Does applying dry palatants on room-temperature fat-precoated kibbles reduce palatant adhesion efficiency? Does this delayed application affect palatability?
  Figure 1: Simplified representation of palatants application in dry pet food.   Experimental Design   Three coating conditions were tested:
  Direct dry palatants application: 3% of fat was sprayed onto 3% fat-precoated kibbles. The 3% fat-precoated kibbles were produced in a standard way, with the fat being sprayed on warm dried kibbles exiting the dryer. There was a three-week interval between the two fat applications, and the latter was followed by an immediate dry palatant application.
  Delayed dry palatants application #1 (3 weeks delay): 6% of fat was sprayed onto uncoated kibbles. After three weeks of storage, the dry palatant was applied.
  Delayed dry palatants application #2 (3 months delay): Same as Condition 2, but with a three-month storage period before applying the dry palatant.   Each condition was tested on both cat and dog kibbles, using dry palatant application levels of 1.5% and 2.0%.
  The same poultry fat (applied at 35 °C) was used across all conditions.   Measurements Recovery Rate (RR): using analytical tracers, the actual percentage of dry palatant recovered on the kibbles was calculated.
  Palatability testing:
carried out in AFB dog and cat kennels with 30 animals over two days,
versus tests were analyzed using paired t-tests for dependent samples.
  Moisture content: controlled to ensure it did not influence palatability results (cat: 5.8% ± 0.1, dog: 7.0% ± 0.1).
  Crude fat content: verified for coating uniformity (cat: 11.9% ± 0.3, dog: 10.4% ± 0.3).   Results   Recovery Rate of dry palatant:
  Figure 2 illustrates the RR of the dry palatant for each condition. Key finding: the RR remained consistent across all conditions, indicating that at this fat level (6%), dry palatants adhesion does not require immediate application after fat coating.   Figure 2: Recovery Rate of dry palatant under different coating conditions.   Palatability Results
Table 1 summarizes the results of the versus tests. 
  Table 1: Palatability test results for differential dry palatants coating (versus test, n = 30 animals).   Key observations
  The tests showed no statistically significant difference in palatability. One case showed a significant preference: cat kibbles with 1.5% dry palatants, applied after 3 weeks.   Conclusion   Delaying dry palatants applications does not reduce adhesion efficiency. Palatability remains unchanged, except in one specific case.   These findings indicate that dry palatants applications can be delayed by days, weeks, or even months after fat coating without compromising product performance or dry palatant adhesion. 
  This flexibility could be particularly useful when:
  Applying dry palatants in a separate coating line (e.g., liquids in a batch coater, powders in a continuous coater). Manufacturing two diets that differ only in the coating step. Conducting trials in AFB facilities using customer-specific fat and application conditions.
  These results are based on a limited set of conditions (one type of fat, one type of kibbles, one dosage of fat) and should not be taken as universally representative. They are intended to provide insights rather than conclusive evidence.
  To learn more about this topic, or to speak with our Science & Technology experts, please reach out to your AFB Sales representative or afbinternational.com/contact.
  By AFB International
Source: All Pet Food Magazine


Proteins

Proteins A to Z of Pet Food: Proteins 
 

2+ MIN

A to Z of Pet Food: Proteins   

Made up of amino acids, proteins build and maintain structures in the body and help support muscles, bones, blood, organs and skin and coat health. Because dogs and cats can't synthesize all the essential amino acids they need, these nutrients must come from complete and balanced pet food.     Sources of Protein    Proteins are commonly classified into two large categories based on their source: animal-based and plant-based.  
  Animal-based protein includes chicken, beef, turkey, lamb and fish.  Plant-based protein includes ingredients like soybeans, corn and lentils. 
  While animal protein sources have higher amounts of essential amino acids, dogs can still thrive on a well-formulated plant-based diet. Cats, on the other hand, are obligate carnivores and rely on animal-based protein for specific amino acids, like taurine, methionine and cystine.    How Much Protein Does My Pet Need?    The Association of American Feed Control Officials (AAFCO) has set the following minimum protein requirements for pet food: 
  Dogs   Adult: ≥ 18% crude protein  Puppy: ≥ 22% crude protein  Cats   Adult: ≥ 26% crude protein   Kitten: ≥ 30% crude protein 
  Puppies and kittens require a higher amount of protein in their diet to support growth and development. Additionally, cats need more protein than dogs and have more specific amino acid requirements. 
  Protein is an essential nutrient for dogs and cats, and diets containing more than the minimum required amount of protein are often beneficial. In fact, in obese dogs, high-protein, low-carb diets can promote both proper body condition and steadier blood glucose levels. Additionally, studies suggest that aging pets likely have a higher dietary protein requirement than younger pets.   
  That said, extremely high levels of protein, especially when not balanced with other nutrients, may pose health risks, such as disrupting a pet's calcium to phosphorus ratio or contributing to weight gain. Consult with your veterinarian to ensure your pet's protein intake matches their unique needs, including life stage, size and activity level.    Looking Ahead: Emerging Protein Alternatives    Did you know over 93% of pet food products worldwide contain animal protein? Although these are most often responsibly sourced as by-products, as the global population grows, so will the demand for animal protein—raising concerns about demand outpacing supply. 
  To ensure the long-term sustainability of pet food, industry research has turned toward alternative sources of protein, including insect-based ingredients, invasive species (such as Asian carp and blue catfish) and single-cell ingredients (like algae, fungi and yeast).  
  Another alternative protein source includes meat products not commonly consumed by humans, like alligator, kangaroo and rabbit. While these alternative ingredients are still being evaluated, they could play a key role in supporting a pet's complete and balanced diet in a more sustainable way.  Source: Pet Food Institute

Other microingredients Gut Helath: What Does Science Say About the Microbiome and the Role of Plasma in Pets?

4+ MIN

Gut Helath: What Does Science Say About the Microbiome and the Role of Plasma in Pets?

Today, the intestine is recognized as a key axis connecting digestion, immunity, and overall well-being. As a result, the microbiome has become one of the main drivers of innovation in animal nutrition.
  At APC, this focus on gut health is not a recent trend but rather the natural evolution of decades of research in nutrition. The company specializes in functional ingredients for animal nutrition and began its work with plasma-derived functional proteins in livestock production, where the link between gut integrity, immunity, and performance is well documented. Over time, this expertise has been transferred to the pet food sector, where the microbiome is now understood as a crucial factor in improving quality of life for dogs and cats, even in apparently healthy animals.
  The intestinal microbiome plays a key role in digestive, immune, and metabolic health, directly impacting overall well-being.  
The Gut: A Key Ecosystem for Whole-Body Health
The gut is not just a digestive organ. It is a complex ecosystem where nutrients, microbiota, and the immune system interact continuously. A significant portion of the body's immune defenses is located in the intestine. Therefore, any imbalance in the microbiota can have effects that extend well beyond digestion.
  Disruptions in this ecosystem are associated with recurring digestive disorders, poor stool quality, or food intolerances. Moreover, they are linked to inflammatory processes that may affect skin condition, immune responsiveness, and overall metabolism. Increasing scientific evidence confirms that maintaining a balanced microbiome is essential for long-term systemic stability.   Plasma: A Functional Protein with Benefits Beyond Nutrition
Within this context, plasma has established itself as a high-value functional ingredient. It is a nutrient-dense protein obtained from surplus blood collected at facilities approved for human consumption. Through a carefully controlled spray dried process, its functional components are preserved.
  Unlike many conventional protein sources used in pet nutrition, plasma provides not only amino acids but also bioactive compounds such as immunoglobulins, growth factors, and functional peptides. Many of these components are similar to those found in milk or colostrum. They act directly at the intestinal level, supporting digestion and nutrient absorption while helping reduce metabolic stress and inflammation.
  Research shows that plasma promotes a more beneficial digestive and fermentation pattern in the gut. Diets containing plasma lead to increased production of short-chain fatty acids, key compounds for intestinal health. At the same time, they reduce digestive by-products associated with intestinal stress.   Supporting the Microbiome Without Compromising Diversity
In addition, plasma modulates the microbiota gradually. It promotes bacterial populations associated with improved intestinal health while maintaining microbial diversity, which is essential for ecosystem stability.
  Importantly, this effect goes beyond digestion. The intestine acts as a systemic regulator of the body. By improving intestinal integrity and supporting mucosal immunity, the overall inflammatory load can be reduced. Consequently, improved gut health may positively influence other tissues and systems, including those involved in chronic inflammatory processes such as joints.
Scientific Evidence Applied to Pet Food
To generate specific scientific evidence for the pet food sector, APC recently supported a study focused on healthy adult dogs fed extruded diets, the most common format in dry pet food.
  The study, conducted in collaboration with the University of Illinois, was published in the Journal of Animal Science under the title:
Spray dried plasma promotes intestinal health and modulates fecal microbiota and metabolites in healthy adult dogs.
  This approach is particularly relevant because it evaluates plasma not as a corrective ingredient but as a functional component that supports balance and optimal physiological function under normal conditions.
  The conclusions are clear. Plasma inclusion:
  Promotes more efficient digestion Increases beneficial intestinal metabolites Reduces digestive by-products associated with intestinal stress Helps balance the microbiota by promoting beneficial bacteria Strengthens natural intestinal defenses without inducing inflammation Improves overall diet digestibility, with effects directly related to inclusion level
  In practice, the first visible improvements often appear in stool quality and regularity. Over time, microbiome balance contributes to greater digestive resilience and more stable immune responses.
  Rather than positioning this approach strictly in preventive clinical terms, it aligns with functional nutrition. The goal is to maintain physiological balance and reduce the risk of future imbalances.   The Future of Functional Pet Nutrition
This nutritional strategy is particularly relevant for pets with sensitive digestion, senior animals, highly active dogs, or those exposed to stress. However, the observed benefits in healthy adult dogs suggest that plasma can also form part of a long-term nutritional strategy.
  Pet nutrition is evolving. It is no longer viewed solely as nutrient supply but as a tool to support specific physiological functions.
  Looking ahead, nutrition will become increasingly personalized, evidence-based, and driven by functional ingredients acting on the gut–microbiome–immunity axis. Ingredients such as plasma, with demonstrated functionality, are redefining the future of animal nutrition.
  Innovation will not come only from new recipes. It will come from understanding how ingredients interact with animal physiology to deliver measurable, sustainable benefits over time. Source: APC


Formulation

Formulation Precision Innovation in Pet Food

4+ MIN

Precision Innovation in Pet Food

Innovation in pet food is an evolving topic driven by trends, including pet humanization, sustainability, and the pursuit of improved nutrition. Innovations in pet food production and processing aim to enhance specific nutrition, functional health, and digestive performance by using the right amount of bioactive compounds and nutrients. We will address this issue from the perspective of ingredient innovation.
  Protein is one of the most important compounds for pet parents when purchasing pet food. Innovation is not the exception to this nutrient in the formulation process. Animal proteins (chicken, lamb, fish, etc.) are the most commonly used, but there are some alternatives. Moreover, others are in the initial implementation phase or used only in a few segments: 
  Insect proteins: Cricket flour, black soldier fly larvae, etc. are highly digestible, sustainable sources, rich in proteins and with less environmental impact.
  Vegetable proteins: Different from traditional sources, such as soybean paste. Nowadays, it is not surprising to see lentils, chickpeas, peas, and/or quinoa, among others.
  Cell culture: Cultured meat for super-premium food is being studied.
  Emerging functional ingredients are another important aspect of innovation in nutrients. They are not only nutritious but also beneficial for health. Sometimes, they result in superfoods that are detailed below:
  Spirulina, chia seeds, and blueberries were widely used in the pet food industry due to their antioxidant and anti-inflammatory benefits.  
  Prebiotics, such as FOS (fructooligosaccharides), MOS (mannan oligosaccharide), XOS (xylooligosaccharides), and beta-glucans are known as gastrointestinal microbiome enhancers that improve the growth of beneficial intestinal bacteria. 
  Probiotics are microorganisms that help the digestive system and the gut microbiome. Innovation in pet food is associated with probiotic capsules or spore-forming probiotics (e.g., Bacillus coagulans, Enterococcus faecium) which support extrusion thermal processing. 
  Postbiotics are by-product metabolites from beneficial bacteria that promote gut and immune health. They also reduce inflammation without needing living organisms.
  Enzymes, such as amylase, protease, and lipase are added to enhance nutrient digestibility, especially in diets with alternative ingredients. However, you should be careful since enzymes are thermolabile and need accurate technology to withstand the thermal process, for example, the extrusion. 
  Organic minerals are more bioavailable than traditional sources (sulfate or oxides); they strengthen metabolism, bone health, dermal health, and the immune system. In this segment, there are different organic sources, depending on the metal-binding element. Beginning with the most advanced technology, these can be bisqueled with HMTBa [(hydroxymethyl)butanoic acid]; followed by chelates, essential and non-essential amino acids, complex polysaccharides, or complex propionic acids (according to the AAFCO's classification).
  Vitamins are essential nutrients for pets' health and well-being. Food and vitamin capsules are typically combined in the pet food industry because they protect from heat and oxidation during the extrusion or cooling processes, improving the final product's stability.
  Phytonutrients and/or botanical extracts, such as polyphenols, flavonoids, and essential oils (e.g., rosemary, turmeric, and oregano) are an alternative due to their antioxidant, anti-inflammatory, and natural preservative properties. 
  Specific fatty acids can be a good source of polyunsaturated fatty acids or omegas. The controlled incorporation of EPA/DHA (omega-3) microalgae as a more sustainable ingredient than fish oil is not surprising. Medium-chain fatty acids are widely used as immediate sources of energy.
  Bioactive peptides are hydrolyzed protein by-products, for example, hydrolyzed collagen with specific functions (immune response modulator, joint health enhancers, cartilage regeneration, and skin elasticity). 
  Functional fermented vegetable foods, such as miso or kefir (adapted for pets), are beneficial as they provide natural digestive enzymes and beneficial microorganisms.
  Adaptogens (e.g., ashwagandha, ginseng, or rhodiola) are natural ingredients that help the body manage stress. Usually, they are used in supplements to reduce anxiety, support the nervous system, and adapt to changes. 
  On the other hand, there is a trend to reduce toxic ingredients in food; in those cases, we must look for innovative alternatives.
  Reduce sodium, sugar, and synthetic additives by replacing artificial colorants with natural pigments (e.g., beta-carotenes, spirulina) or substituting artificial antioxidants with natural products. 
  Referring to precision innovation, we cannot forget about integrating technology, such as apps that recommend diets or provide nutritional follow-up or intelligent vending machines that ration food and compile consumption data. 
  Another innovative area we must follow closely is innovation in formulation and dosing. Microencapsulation technologies are still evolving to enhance the stability of sensitive compounds (vitamins, oils, and probiotics). Some lab tests allow the understanding of certain genetic predispositions and the gut health of our pets. The applied nanotechnology area is being developed, related to the use of nanoparticles that improve the absorption of minerals and antioxidants (even in the experimental or regulatory stage). Finally, data-driven formulation employs AI and nutritional algorithms that adjust the right amount of micro-ingredients according to age, breed, activity or pathologies, sleep, and pet food consumption in real time. 


By MVZ Armando Enriquez de la Fuente Blanquet
Source: All Pet Food Magazine

By Armando Enriquez de la Fuente Blanquet

Formulation From Claim to Credibility: The Case for Research-Backed Pet Food Ingredients

4+ MIN

From Claim to Credibility: The Case for Research-Backed Pet Food Ingredients

The pet food industry is unique in that of all the 602 standard pet food ingredients used today, 44% of them are upcycled, which means that they are derived from animal and plant sources that would not be used in human food. This makes pet food a great source to utilize ingredients that otherwise would be wasted, and reduces the competition between human food and pet food for ingredients that span both categories.
  This becomes increasingly important as currently 9% of the world's population is considered hungry, according to a recent report by the World Health Organization. Unfortunately, these upcycled ingredients may not be viewed as premium ingredients by consumers who have decided that descriptions such as 'by-product' are not good for their pets. Many times, these decisions are not based on nutritional research but on messaging from pet food companies. Starches, flours, and functional dietary fibers have been described by many pet food brands as 'fillers,' when there actually is no definitive or regulatory definition for that term. In most cases, these ingredients not only provide nutritional value for pets but also deliver functional benefits that make the pet food easier to actually manufacture.   
  New pet food ingredients and categories have become more popular. The pet food protein category has seen many new entries and continues to see new types of proteins entering the market. Examples of these proteins include cultured meat, insect-based, bacteria- and yeast-based cultures, and upcycled food ingredients. All these new and novel pet food proteins collectively have the potential to help decrease the competition of using animal-based proteins in pet food that could be used in the human food supply. Each one has valid reasons for use, including sustainability, low carbon footprint, as well as delivering needed alternative protein sources for pet food in a competitive landscape. 
  Beyond protein, it is well accepted that Omega-3 fatty acids can help deliver benefits such as skin and coat support, joint health, cognitive health, as well as immune benefits to pets. Most of the Omega-3 fatty acids in pet food, such as EPA and DHA, come from fish oil. As with animal protein ingredients, there is higher competition for fish oil products in human goods when compared to pet food and pet supplement use. Microalgae products have become more prevalent in the pet food world, being described as more sustainable alternatives to fish oil and helping to decrease the reliance on fish oil to deliver the health benefits of Omega-3 fatty acids. 
  Another new trend in the past few years is the promotion of postbiotics. These are inactivated microorganisms, such as yeast or bacteria, and their components that are released after probiotics are no longer alive, which have many health benefits, including digestive health, immune support, and even oral health benefits for pets. Another claimed benefit is that they do not require live bacteria like probiotics do, which may make them more user-friendly in pet food manufacturing.
  These are all examples of new pet food ingredients and categories. Many of these novel pet food ingredients have the potential to help deliver functional benefits to our pets, as well as contribute to the sustainability of the pet food industry as a whole. However, when it comes to the business of the pet food industry, there is a lot of competition in the ingredient space. Many of these newer ingredients come at a premium price when compared to their traditional ingredient counterparts. This means that even though pet food brands may want to use them in products because of the benefits and claims they provide, they have to be affordable and must deliver value that the pet food company and consumer understand. 
  A good way to determine the value of an ingredient is by looking at the extent of research that has been done by the ingredient company to substantiate the claims they promote. Pet food ingredient companies should be conducting feeding study research to validate the claims they make. In vitro (laboratory) studies are helpful for screening tests. However, to actually demonstrate ingredient value, the pet food ingredient industry should be conducting feeding studies to show health benefits in pets. Long-term feeding studies using new ingredients and measuring health parameters are sorely needed to gain credibility for ingredient companies. 
  Ingredient companies should be working with experts in nutrition, physiology, and veterinary medicine, such as those at BSM Partners, to design, implement, and interpret clinical research to help substantiate claims and deliver the value that pet food brands and consumers deserve. After all, if ingredient suppliers and pet food brands don't do feeding studies with their products before they hit the market, pet parents' companions become the defacto study subjects and nobody wants that.


By Bradley Ques
Source: BSM Partners

About the Author
Dr. Bradley Quest, DVM, is the Principal Veterinarian at BSM Partners. He has practiced clinical veterinary medicine, developed and tested hundreds of pet food and health products, performs extensive animal health research, and helps navigate pet food ingredient approval for clients.

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