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Rethinking Protein in Pet Nutrition: From Traditional Sources to Alternative and Functional Proteins
Proteins

8+ MIN

Rethinking Protein in Pet Nutrition: From Traditional Sources to Alternative and Functional Proteins

In recent years, however, the discussion around protein has expanded significantly. Terms such as 'alternative proteins', 'novel proteins', and 'functional proteins' are increasingly used across the food and pet food industries. Much of this interest is driven by sustainability concerns and projections that global demand for protein will increase substantially as the human population approaches 10 billion by 2050 (FAO, 2022; OECD-FAO, 2023).
  This raises an important question: do we truly need entirely new protein sources to feed our pets, or should we focus on improving how we utilize the protein sources we already have?
  The answer likely lies somewhere in between. Traditional protein sources remain nutritionally robust, while emerging technologies and alternative ingredients offer opportunities to improve sustainability, functionality, and health outcomes—including effects on the gut microbiome.
Global Protein Demand and the Sustainability Debate   Projections toward 2050 suggest that global protein demand will rise sharply as the world population approaches 10 billion, with food demand expected to increase by about 60% (FAO 2018; FAO 2022; Henchion et al., 2017). At the same time, the global pet population continues to expand, with an estimated 900 million dogs and cats worldwide. Approximately 60% of these animals are concentrated in Europe, the United States, China, and Brazil.
  These trends have raised concerns about whether traditional livestock production alone can meet future protein needs. Critics often argue that feeding animal-derived proteins to pets competes with the human food supply and contributes to environmental pressures, such as land use, greenhouse gas emissions, and water consumption.
  However, the picture is more nuanced. A significant portion of animal-derived ingredients used in pet foods originates from rendered co-products of the human food system, including organ meats, trimmings, and fish processing residues. These ingredients represent an efficient form of nutrient recycling within the food supply chain (Boland et al., 2013).
  Advances in food processing technologies are also improving nutrient utilization, enabling greater recovery of proteins, peptides, and bioactive compounds from raw materials and by-products (Yuan et al., 2025). Improving how proteins are processed and utilized may therefore be as important as identifying entirely new protein sources.   Protein Requirements in Dogs and Cats   Protein requirements in companion animals are well established through decades of nutritional research. According to AAFCO guidelines (AAFCO 2026), the minimum protein requirement for adult dogs is approximately 18% on a dry matter basis, while growing puppies require 22.5%. Cats, as obligate carnivores, require higher protein intake, with minimum levels of 26% dry matter.
  In commercial diets, however, protein levels are typically higher. Many dry dog foods contain 22–32% protein, while cat foods often range from 30–40% protein. These levels reflect both nutritional needs and consumer expectations, as protein content has increasingly become associated with perceived diet quality.   Traditional Protein Sources: Still the Foundation   Animal-derived proteins remain central to pet nutrition because of their balanced amino acid profiles and high digestibility. Common ingredients include chicken meals, beef and lamb meals, fish meals, fish protein concentrations, and meat or organ by-products. These ingredients supply essential amino acids, such as lysine, methionine, and taurine and typically exhibit digestibility values around 85–90%, although digestibility can vary depending on the specific ingredient and processing conditions.
  Plant proteins also play a significant role in modern formulations. Ingredients such as soybean meals, pea protein, lentils, and chickpeas contribute valuable amino acids while offering flexibility and cost efficiency in formulation.
  Recent innovations in food processing have further improved plant protein functionality. Technologies such as fermentation, enzymatic hydrolysis, and advanced milling can increase digestibility while reducing anti-nutritional factors such as lectins and protease inhibitors (Yuan et al., 2025).
  These developments highlight an important point: innovation does not always require entirely new ingredients—sometimes it involves improving how existing ingredients are processed and utilized.   The Rise of Fresh and Minimally Processed Meat Ingredients   Over the past decade, the use of fresh meat or poultry has expanded significantly in dry pet food formulations. Many premium kibble products now incorporate fresh chicken, beef, or fish as part of their protein systems.
  In many cases, these fresh ingredients originate from mechanically deboned meat (MDM) or automated meat recovery (AMR) processes, which efficiently recover edible muscle tissue from carcasses after primary cuts have been removed. These ingredients provide highly digestible protein while improving palatability and consumer perception.
  Marketing narratives around 'fresh' ingredients have also been reinforced by the rapid growth of fresh and lightly cooked pet food formats. The fresh pet food market in the United States alone is estimated to exceed USD 3 billion and continues to grow (Packaged Facts, 2023). This trend illustrates how consumer expectations increasingly influence ingredient selection and processing strategies in the pet food industry.   The Rise of Alternative Proteins   While traditional proteins remain dominant, several emerging protein technologies are gaining attention.
  Insect Proteins
Insects, such as black soldier fly larvae, mealworms, and crickets are being explored as sustainable protein sources. Insect meals typically contain 40–65% protein and can be produced using relatively small amounts of land and water compared with conventional livestock production (van Huis, 2021).
  Insect proteins may contain bioactive compounds such as antimicrobial peptides and chitin that could influence immune function and gut health (Gasco et al., 2020). However, scaling production and achieving widespread consumer acceptance remain challenges.   Fermentation-Derived Proteins
Fermentation technologies represent another promising pathway. Microbial fermentation can produce single-cell proteins (SCP) using yeast, bacteria, fungi, or microalgae grown on various substrates (Matassa et al., 2016). These proteins can provide favorable amino acid profiles while requiring relatively small land footprints.
  Traditional fermentation methods—such as koji fermentation using Aspergillus oryzae—can also transform plant substrates into more digestible and nutritionally enhanced ingredients (Yuan et al., 2025). Advances in biotechnology now allow engineered microbes to produce specific peptides or proteins with targeted functional properties.   Cell-Cultured Proteins
Cellular agriculture represents one of the most technologically ambitious approaches to protein production. By culturing animal cells in controlled environments, it may eventually be possible to produce meat without traditional livestock production (Post, 2012). Although promising, this technology is still in its early stages—particularly for pet food applications—and faces challenges related to cost, energy use, and regulatory frameworks.   Protein and the Gut Microbiome
One of the most exciting areas linking protein nutrition and health is the gut microbiome.
  The gut microbiota plays a central role in digestion, immune function, and metabolic health in companion animals. Diet composition—including protein source and digestibility—can significantly influence microbial communities in the gastrointestinal tract (Handl et al., 2013).
  Highly digestible proteins reduce the amount of undigested nitrogen reaching the colon, minimizing the production of undesirable fermentation by-products, such as ammonia or biogenic amines.
  Conversely, certain peptides and amino acids may serve as substrates for beneficial microbial populations, contributing to the production of short-chain fatty acids and other metabolites that support intestinal health (Sandri et al., 2017).   Key Takeaways   The conversation around alternative proteins often frames the issue as a replacement of traditional proteins. Actually, the future of protein in pet nutrition will likely be more integrated and from this discussion, several key insights emerge:
  Traditional protein sources remain nutritionally efficient. Animal and plant proteins continue to provide reliable amino acid nutrition and will remain foundational ingredients in pet food.
  Alternative proteins expand the toolbox; insects, microbial fermentation, and cellular agriculture may complement existing protein systems.
  Processing technology and advances in fermentation, enzymatic modification, and ingredient recovery can improve nutrient utilization, reduce anti-nutritional factors, and enhance the functional value of protein ingredients.
  Consumer expectations are reshaping protein systems. The growth of fresh, minimally processed, and high-protein pet foods illustrates how market trends increasingly influence ingredient selection and processing technologies.
  Ultimately, the goal should not be to replace traditional proteins with alternatives but to develop a diverse and efficient protein ecosystem that supports sustainability, nutrition, and pet health. The key question is not only which proteins we choose, but how intelligently we use them. By Juan Gómez-Basauri, Ph.D. - MAGELLAN LLC
Source: All Pet Food Magazine   References
AAFCO 2026. Association of America Feed Control Officials. Official Publication Boland, M., Rae, A., Vereijken, J., Meuwissen, M. P. M., Fischer, A. R. H., van Boekel, M. A. J. S., Rutherfurd, S. M., Gruppen, H., Moughan, P. J., & Hendriks, W. H. (2013). The future supply of animal-derived protein for human consumption. Trends in Food Science and Technology, 29(1), 62-73.https://doi.org/10.1016/j.tifs.2012.07.002 FAO. 2018. The future of food and agriculture – Alternative pathways to 2050. Summary version. Rome. 60 pp. Licence: CC BY-NC-SA 3.0 IGO.https://openknowledge.fao.org/handle/20.500.14283/i8429en FAO. 2022. The future of food and agriculture – Drivers and triggers for transformation. The Future of Food and Agriculture, no. 3. Rome.https://doi.org/10.4060/cc0959en Gasco, L., Gabriele Acuti, Paolo Bani, Antonella Dalle Zotte, Pier Paolo Danieli, Anna De Angelis, Riccardo Fortina, Rosaria Marino, Giuliana Parisi, Giovanni Piccolo, Luciano Pinotti, Aldo Prandini, Achille Schiavone, Genciana Terova, Francesca Tulli & Alessandra Roncarati (2020) Insect and fish by-products as sustainable alternatives to conventional animal proteins in animal nutrition, Italian Journal of Animal Science, 19:1, 360-372.https://www.tandfonline.com/doi/pdf/10.1080/1828051x.2020.1743209 Handl S., German AJ, Holden SL, Dowd SE, Steiner JM, Heilmann RM, Grant RW, Swanson KS, Suchodolski JS. Faecal microbiota in lean and obese dogs. FEMS Microbiol Ecol. 2013 May;84(2):332-43. Epub 2013 Jan 24. PMID: 23301868.https://doi.org/10.1111/1574-6941.12067 Henchion, M. Hayes M, Mullen AM, Fenelon M, Tiwari B. Future Protein Supply and Demand: Strategies and Factors Influencing a Sustainable Equilibrium. Foods. 2017 Jul 20;6(7):53.https://pmc.ncbi.nlm.nih.gov/articles/PMC5532560/ Matassa, S., Boon N, Pikaar I, Verstraete W. Microbial protein: future sustainable food supply route with low environmental footprint. Microb Biotechnol. 2016 Sep;9(5):568-75. Epub 2016 Jul 8.https://pmc.ncbi.nlm.nih.gov/articles/PMC4993174/pdf/MBT2-9-568.pdf OECD/FAO 2023. OECD-FAO Agricultural Outlook 2023-2032, OECD Publishing, Paris.https://doi.org/10.1787/08801ab7-en. Post MJ. Cultured meat from stem cells: challenges and prospects. Meat Sci. 2012 Nov;92(3):297-301. doi: 10.1016/j.meatsci.2012.04.008. Epub 2012 Apr 11. PMID: 22543115.https://doi.org/10.1016/j.meatsci.2012.04.008 Sandri, M., Dal Monego S, Conte G, Sgorlon S, Stefanon B. Raw meat-based diet influences fecal microbiome and end products of fermentation in healthy dogs. BMC Vet Res. BMC Vet Res 2017; 13 (1):65.https://pmc.ncbi.nlm.nih.gov/articles/PMC5331737/ van Huis, A. Prospects of insects as food and feed. Org. Agr. 11, 301–308 (2021).https://doi.org/10.1007/s13165-020-00290-7 Yuan, Yi, Xinyao Wei, Yuhong Mao, Yuxue Zheng, Ni He, Yuan Guo, Ming Wu, Joseph Dumpler, Bing Li, Xu Chen, Xixi Cai, Jianping Wu, Yongqi Tian, Sihan Xie, Jeyamkondan Subbiah, Shaoyun Wang. Innovative Food Processing Technologies Promoting Efficient Utilization of Nutrients in Staple Food Crops, Engineering, Volume 50, 2025, Pages 229-244.https://doi.org/10.1016/j.eng.2025.04.014

The Protein Matrix: Balancing Formulation, Operational Realities, and Consumer Demands
Proteins

5+ MIN

The Protein Matrix: Balancing Formulation, Operational Realities, and Consumer Demands

By Felipe Martínez R.

Today, as pets are increasingly humanized and elevated to the status of family members, the scrutiny placed on the ingredients in their bowls has never been higher. This is why, for this edition, I'm taking a different approach from my past articles and decided to write about the most scrutinized, expensive, and dynamic component of any pet food formula: protein.

The true value of a raw material is no longer dictated solely by its price per ton. Instead, it is defined by a complex matrix of nutritional efficacy, marketing appeal, and manufacturing viability. The universe of ingredients is expanding rapidly, introducing alternative proteins, functional extracts, and novel additives. However, to truly understand how these ingredients deliver value, we must examine how the menu of ingredients, including the different protein sources, complement each other in three dimensions: nutrition, consumer trends, and the realities of managing these materials on the factory floor.
Nutrition: How Proteins Complement Each Other
From a purely nutritional standpoint, a pet does not require specific ingredients; it requires specific nutrients. The goal of any formulator is to deliver a complete and balanced amino acid profile, alongside high digestibility and bioavailability, and palatability—because if the pet does not eat the product, all the effort has been done for nothing. Achieving this is rarely accomplished efficiently with a single protein source. Instead, formulation is all about complements.

Traditional animal protein sources, including chicken, beef, fish, and lamb meal, have long been the workhorses of the industry, offering high protein density and excellent palatability. However, they are often complemented with each other and with plant-based options, such as peas, soybeans, potatoes, or corn gluten meal.

Why blend them?  Because what one ingredient lacks, another provides. For instance, plant-based proteins can be highly digestible and lower the overall ash content of the formula, but they may be limited in essential amino acids (methionine or taurine precursors). By strategically pairing it with a marine protein source, such as salmon meal or a functional extract like yeast, a formulator can bridge the amino acid gap while maintaining a specific price point and functional target.

Furthermore, the rise of alternative proteins, including insects, single-cell proteins, and cultivated meats, is altering the formulation landscape. These ingredients are not just novelties; they can offer functional benefits, such as hypoallergenic properties or high levels of antimicrobial peptides. They 'play' within a formula, not just as bulk protein, but as functional additives that elevate the nutritional profile and the true value of the end product.
The Marketing Push: Premiumization and the Clean Label
While formulators focus on amino acids, digestibility, bioavailability, and palatability, the focus of marketing departments and consumers is the ingredient list and the product claims. In today's premium and super-premium segments, communication trends heavily influence product design, sometimes at odds with traditional formulation logic.

The push for 'clean label' products and limited ingredient diets is a prime example.  Higher-value segments are demanding shorter ingredient lists, driven by a consumer perception that fewer ingredients equate to a more natural, wholesome, and transparent product. This creates a significant challenge: how do you deliver a perfectly balanced amino acid profile when your marketing brief restricts you to a single protein source and a single carbohydrate source?

This restriction has driven the industry toward novel proteins. These new ingredients, such as rabbit, kangaroo, wild boar, herring, venison, and duck, are commanding premium prices—they serve a dual purpose. First, they are highly effective for pets with suspected food sensitivity or allergies to common proteins like poultry or beef. Second, they provide a powerful narrative for marketing and communication. Novel proteins instantly differentiate a brand on a crowded retail shelf, communicating exclusivity and premium quality.

However, the true value here also hinges on the traceability of origin. Consumers put value into knowing that the rabbit was sustainably sourced or if the salmon was wild. Traceability has evolved from a supply chain buzzword into a non-negotiable consumer demand and a core pillar of a brand's value proposition. The story behind the ingredient is now just as important as the ingredient itself.
The Factory Floor Reality: Navigating Production Pain Points
A recipe can look perfect on paper and test brilliantly in a consumer focus group, but it must be feasible to manage within an existing manufacturing process. Managing diverse and novel proteins in a high-volume factory introduces pain points that question the concept of "true value."

One of the most persistent challenges is managing the natural variability of raw materials, particularly animal by-product meals. Meat and bone meals, for instance, can vary significantly from batch to batch depending on the rendering process and the exact source materials. A classic factory pain point is color variation. If a specific batch of chicken meal contains a higher concentration of blood, the resulting kibble will be noticeably darker. While the product remains entirely safe and nutritionally sound, this visual inconsistency can inevitably lead to consumer complaints. Pet owners are conditioned to expect absolute uniformity, and a dark batch of kibble is often misinterpreted as burnt or spoiled. Managing this requires strict supplier specifications, advanced mixing techniques, and sometimes, the reluctant use of colorants to standardize the final appearance.

Beyond color, different proteins behave differently during extrusion. Plant proteins often require different specific mechanical energy and moisture inputs compared to animal proteins. High levels of fresh meat, while highly appealing on an ingredient label, introduce massive amounts of water into the formula, which must be managed to ensure the kibble expands properly and dries to a safe moisture level to prevent mold.
Conclusion: Redefining True Value
The universe of pet food ingredients is undoubtedly expanding, but 'true value' is not found simply by adding the newest, trendiest protein to a formula. True value is achieved at the intersection of three distinct disciplines.

It requires the formulator's skill to blend complementary amino acid profiles for optimal animal health. It demands the marketer's insight to select ingredients that resonate with consumer demands for transparency, limited ingredients, and sustainable origins. And crucially, it relies on the factory expertise to handle the physical realities, variabilities, and organoleptic challenges of processing raw materials at scale.

As the industry continues to innovate beyond price, the brands that succeed will be those that master this complex matrix, ensuring that every raw material earns its place in the bowl: nutritionally, commercially, and operationally. By Felipe Martinez R.
Source: All Peto Food Magazine

Black Soldier Fly Larva Meal: A Sustainable and Functional Protein Source for Pet Food
Proteins

7+ MIN

Black Soldier Fly Larva Meal: A Sustainable and Functional Protein Source for Pet Food

By Josiane Volpato

A New Scenario for Proteins in the Pet Food Industry   In this scenario, the pet food industry has been looking for alternatives that simultaneously meet nutritional, technological, and environmental requirements, promoting innovation without compromising the quality of the final products.
  Among the main trends, the partial or total replacement of traditional protein sources, such as poultry viscera meals, meat and bone meal, fish meal, and soybean meal, with more sustainable ingredients with less environmental impact stands out. In this context, black soldier fly larva meal (Hermetia illucens or BSF) has gained prominence as a promising solution to the current challenges of the production chain.
  The production of this ingredient is directly related to the ability of the larvae to convert organic waste into biomass of high nutritional value. This process, known as bioconversion, allows the use of agro-industrial by-products, reducing waste and contributing to circular economy models. In this way, BSF flour not only meets nutritional demands but is also part of a broader sustainability strategy in the pet food sector.
  From a nutritional point of view, BSF flour has a variable protein content, usually between 35% and 60%, depending on the substrate used in the larvae and the industrial processing applied. Although this content is considered intermediate compared to some conventional sources, the ingredient stands out for the quality of its protein and amino acid profile, suitable for the nutritional requirements of dogs and cats.
  In addition, BSF flour has a high lipid content, especially lauric acid, a medium-chain fatty acid associated with antimicrobial properties and potential benefits to intestinal health. This differentiated lipid composition can contribute not only to the energy value of diets but also to important functional effects in the animals' bodies.     Recent studies also indicate additional benefits associated with the use of BSF flour in extruded diets, including improvements in skin integrity, coat quality, and antioxidant response. These effects are possibly related to the presence of bioactive compounds and the quality of the nutrients present in the ingredient.
  Another relevant point is the presence of minerals, such as calcium and phosphorus, at significant levels, as well as essential amino acids, e.g., methionine and tyrosine. The higher concentration of tyrosine, in particular, has been highlighted as a differential of BSF flour, reinforcing its nutritional potential in complete formulations.
  In addition, BSF flour contains compounds with potential prebiotic and antibacterial action, which can contribute to the modulation of gut microbiota and the maintenance of digestive health. This set of characteristics makes the ingredient attractive not only as a source of protein but also as a functional component in diets for dogs and cats.
  Because it is an alternative protein and still little used on a large scale, BSF flour also has potential application in hypoallergenic diets, being an interesting option for animals with food sensitivities to conventional proteins, such as chicken, beef or soy.   Digestibility: The Critical Point of Formulation for a Sustainable Future in Pet Food   Digestibility is one of the main criteria for evaluating the quality of new ingredients in animal nutrition. In this aspect, BSF flour presents consistent results, with protein digestibility coefficients similar to those observed in diets formulated with traditional ingredients.
  Studies indicate that inclusion levels ranging from 5% to 20% result in apparent protein digestibility of 83% to 84%, values compatible with those observed in conventional diets. These results demonstrate that the ingredient is efficiently used by the animals' bodies, without compromising fecal quality.
  In addition to the protein fraction, the lipid digestibility of BSF flour is also noteworthy, being frequently high due to the presence of medium-chain fatty acids, which are easier to digest and absorb when compared to long-chain fatty acids.
  The presence of chitin, a structural polysaccharide in the exoskeleton of larvae, represents another important aspect from a nutritional point of view. Chitin can act as a functional fiber, contributing to the modulation of the gut microbiota and the formation of stools with better consistency. However, its effect depends directly on the level of inclusion and processing of the ingredient, and can, at high concentrations, interfere with the digestibility of nutrients.
  Thus, the use of BSF flour requires an adequate balance in the formulation of diets, in order to maximize its functional benefits without compromising nutritional use.     Palatability and Acceptance by Animals    Another determining factor for the practical application of the ingredient is palatability. The acceptance of food by animals is one of the main indicators of commercial success, especially in diets for cats, which have more selective feeding behavior.
  In general, the inclusion of BSF flour does not compromise the intake, and good acceptance is observed in both dogs and cats. However, studies indicate that this factor is strongly dependent on the level of inclusion.
  Lower levels of substitution, such as 3%, maintain intake similar to the control diet and may even stimulate the animals' initial interest in the food. On the other hand, higher levels, such as 6%, tend to reduce intake and preference. This effect may be associated with the lipid composition of the larvae, especially the higher content of medium-chain fatty acids, which can influence the sensory perception of food.
  Thus, the definition of the appropriate level of inclusion is essential to ensure the acceptance of the final product, with moderate inclusion being the most suitable strategy for commercial formulations.
  In addition to the nutritional and functional aspects, BSF flour has significant environmental advantages when compared to traditional protein sources. Its production requires less use of natural resources, such as water and agricultural areas, in addition to resulting in lower greenhouse gas emissions.
  The ability of larvae to convert organic waste into high-value protein allows the use of by-products that would otherwise be discarded, contributing to the reduction of the environmental impact of the production chain.
  Another important point is production efficiency. Insect farming has better feed conversion and lower need for inputs when compared to conventional livestock, making it a viable alternative for more sustainable production systems.
  This set of characteristics positions BSF flour as an ingredient in line with the main global trends in the pet food industry, which seeks to integrate nutritional performance, innovation, and environmental responsibility.
  In conclusion, black soldier fly larva meal consolidates as a strategic ingredient for the pet food industry, bringing together nutritional quality, functionality, and environmental benefits. Its use allows the development of more sustainable diets without compromising digestibility, fecal quality, or acceptance by animals, representing a promising solution for the future of dog and cat nutrition. By Bruna Cavalari Santello; Laura Cicília Cassol da Silva; Douglas Melo de Souza; Lorenna Nicole Araújo Santos; Josiane Aparecida Volpato
Source: All Pet Food Magazine
  References
ABD EL-WAHAB, A.; et al. Insect larvae meal (Hermetia illucens) as a sustainable protein source of canine food and its impacts on nutrient digestibility and fecal quality. Animals, Basel, v. 11, n. 9, p. 2525, 2021. BOSCH, G.; VERVOORT, J. J. M.; HENDRIKS, W. H. In vitro digestibility and fermentability of selected insects for dog foods. Animal Feed Science and Technology, v.221, p.174–184, 2016.  CARDOSO, R. K. N. FEDERAL UNIVERSITY OF BAHIA (UFBA). Nutritional evaluation of black soldier fly larva meal in extruded diets for dogs and effect on intestinal health. Salvador: Repositório da UFBA, 20 (thesis/dissertation). 2 Jul. 2024. CARVALHO, L. C.; et al. Possible use of insect meal in the diet of dogs and cats. Caderno de Ciências Agrárias-UFMG, 2016. DE ANDRADE, G. C.; et al. Alternative foods for the formulation of diets for dogs and cats. Animal Science, v. 35, n. 2, p. 45-56, 2025. FREEL, T. A.; MCCOMB, A.; KOUTSOS, E. A. Digestibility and safety of dry black soldier fly larvae meal and black soldier fly larvae oil in dogs. Journal of Animal Science, v. 99, n.3, 15 fev. 2021. GONÇALVES, C. A.; RIBEIRO, G. B.; AFONSO, M. V. R. Assessment of population knowledge about breeding and management of dogs and cats. Veterinária Notícias (Online), v.1, n.1, p.1-11, 2022. KWAKERNAAK, C.; et al. Apparent nutrient digestibility, metabolizable energy and apparent ileal amino acid digestibility of commercial partially defatted Hermetia illucens meal for laying hens. Journal of Insects as Food and Feed, p. 1–12, 8 dez. 2023. LAYNE, C.A. My dog is in teletherapy with me: The impact of a pet dog on their owner's teletherapy session, 2023. 154p. (Tese de Doutorado em Community Care and Counseling). Liberty University, Lynchburg, VA, 2023. Liu, X.; et al. Dynamic changes of nutrient composition throughout the entire life cycle of black soldier fly. PLOS ONE, v. 12, n. 8, e0182601, 2017. Lu, S.; et al. 'Nutritional Composition of Black Soldier Fly Larvae (Hermetia illucens L.) and Its Potential Uses as Alternative Protein Sources in Animal Diets: A Review.' Insects vol. 13,9 831. 13 Sep. 2022. VALDÉS, F. V.; et al. Insects as Feed for Companion and Exotic Pets: A Current Trend. Animals, v. 12, n. 11, p. 1450, 3 jun. 2022. Hyuck Kim, et al. Evaluation of black soldier fly larvae reared on different organic substrates on nutrient digestibility and palatability in cats. J Anim Sci Technol 2025; 67(2):477-488.
PENAZZI, L.; et al. In vivo and in vitro Digestibility of an Extruded Complete Dog Food Containing Black Soldier Fly (Hermetia illucens) Larvae Meal as Protein Source. Frontiers in Veterinary Science, v. 8, 11 jun. 2021.  Silva Carvalho, R. et al. Effect of feeding a diet based on black soldier fly larvae meal on canine skin barrier function, organic antioxidant defense, and blood biochemistry. 2024. Arquivos de Nutrição Animal , 78 (2), 159–176. 

 Leveraging Artificial Intelligence to Predict Pet Food Palatability
Palatants

2+ MIN

 Leveraging Artificial Intelligence to Predict Pet Food Palatability

WHY THIS MATTERS
Palatability is one of the strongest drivers of product success in pet food. Yet, predicting it remains challenging. Development still relies heavily on iterative animal trials, which are time-consuming, costly, and often identify failures late in the process.
  At AFB, we are changing this approach by using artificial intelligence (AI) to predict palatability outcomes before testing. By combining historical data with formulation knowledge, we help transform development from trial-and-error into a more targeted and efficient process.
  THE CHALLENGE
Palatability is the result of multiple interacting factors, including ingredients, inclusion levels, fat systems, processing, and animal variability. These interactions are complex and non-linear, making outcomes difficult to anticipate.
  Because of this complexity, traditional approaches struggle to efficiently explore formulation space and consistently deliver high-performing solution.   A NEW APPROACH: FROM TESTING TO PREDICTING
Instead of relying only on experiments, we integrate data across trials and formulations to identify patterns and predict outcomes.
  Our models transform inputs such as recipe composition, ingredient levels, and processing conditions into clear guidance: which solutions are most likely to succeed and which carry higher risk.
    FROM DATA TO DECISIONS
Our approach converts complex datasets into practical insights that guide development. Rather than focusing only on performance, we also consider how reliable each result is.
  By combining performance with confidence, we generate a clear prioritization of what to test next. This allows teams to focus on the most promising formulations and avoid unnecessary trials.
    WHAT THIS ENABLES

This predictive approach allows us to:
  Identify high-potential solutions earlier Reduce experimental burden Focus on the most impactful ingredients Improve consistency of results
  In practice, large experimental spaces can be narrowed down to a smaller set of high-confidence candidates, accelerating development and improving outcomes.   A SMARTER WAY TO DEVELOP PALATANTS
Artificial intelligence does not replace expertise—it enhances it. Scientific knowledge remains central to interpretation and validation, while predictive models help guide decisions and reduce uncertainty.
  By combining data, science, and AI, we enable a more efficient and confident approach to palatability development. Source: AFB International

From Alga to Bowl: Astaxanthin Supports Pet Health 
Vegetable Origin

5+ MIN

From Alga to Bowl: Astaxanthin Supports Pet Health 

Owing to the ongoing anthropomorphism of pets, owners are taking a closer look at what they're feeding their beloved companions. Just as they themselves are opting for healthier and cleaner-label nutrition—and starting to consider the associated environmental aspects—, owners want to ensure that the same standards apply to their pet's meals. This is manifesting as a shift towards providing fresh food or preferentially selecting more natural or organic pet food products.

Overall, the pet food sector is witnessing continuous growth. The market size in Europe is estimated to be worth $55 billion in 2024 and is expected to reach a value of more than $78 billion by 2029 (growing at a CAGR of approximately 7%).1 Next to classic feed compounds, such as grain or meat, novel functional ingredients are gaining traction in this developing market. Many of these components can be both integrated into feed grades as well as pet-friendly nutraceuticals. One example is carotenoids, which are not only known for their vibrant yellow, orange, or red colours, but also for their antioxidant attributes. One carotenoid with a particularly high antioxidant power—110 times more than vitamin E—is astaxanthin. 

In nature, the most abundant source of astaxanthin is a tiny microalga called Haematococcus pluvialis. Although astaxanthin has a long history as an ingredient in human supplements, its beneficial effects were first discovered in the marine world regarding the survival and reproduction rates of salmon. Research on astaxanthin in aquaculture was also the basis for the Swedish company, AstaReal's business. The organisation was the first to produce natural astaxanthin on an industrial level and, as the subject of more than 70 clinical studies on humans and animals, it owns today's most researched astaxanthin brand in the market.
Species-Specific Research
'We wanted to understand what this might mean for different kinds of animals, so we started using the ingredient in trials with cats and dogs,' says Peter Ahlm, Head of Marketing & Sales at AstaReal. Distinct research on targeted animals is indispensable when it comes to developing a safe and efficacious product; effects might vary between species, and it's imperative to optimise the amount of ingredients to be dispensed.

There is a growing body of evidence for various positive effects of natural astaxanthin on pets' health, many of which show a similar pattern to humans. For instance, astaxanthin can support canine mobility, endurance, and muscle recovery; the cardiovascular system; cognitive function; attentiveness and at the cellular level, the mitochondria.2–5 Moreover, natural astaxanthin supplementation in dogs and cats could enhance their innate defence systems and improve both their cell-mediated and humoral immune responses.6,7 Astaxanthin also has shown potential to improve impaired vision due to age-related conditions like cloudy lenses in dogs.8 

'The recorded health effects may seem quite diverse; however, they are all rooted in astaxanthin's unique molecular structure. Due to its linear, polar-nonpolar-polar molecular layout, natural astaxanthin can effectively penetrate and traverse the cell and mitochondria membranes and neutralize reactive oxygen species (ROS) in both the hydrophobic interior and hydrophilic exterior boundaries. In addition to providing better protection to the cells and their powerhouses against oxidative stress, natural astaxanthin also has anti-inflammatory effects that support multiple bodily organs and systems.' -  Behnaz Shakersain, Scientific Affairs Manager at AstaReal.
Scoring with Natural Astaxanthin Options
As neither humans nor animals can produce astaxanthin in their bodies, they can only benefit from its protective functions through nutritional intake. To obtain effective amounts, dogs are generally recommended to be fed 1 mg of astaxanthin per 10 kg body weight, which equates to about two wild King salmon fillets for a beagle or three for a golden retriever per day. Feed or treats rich in astaxanthin might offer an easier and more sustainable solution in many cases. 

Natural algae-derived versions of astaxanthin offer additional advantages, such as higher levels of antioxidant potency. Moreover, algae play an essential role in the planet's ecosystem, and being recognised as a renewable resource resonates strongly with responsible consumers. If algae are cultivated indoors, they can be better protected from environmental harm or contamination, and the yield of astaxanthin-rich algal biomass has a higher quality profile. 

Developing a formula with healthy ingredients is only the first step, according to Peter Ahlm: 'Pet food manufacturers also need to make sure that their product will remain stable and nutritious throughout its intended shelf-life. Bioactive substances are particularly prone to interact with other compounds in multi-ingredient formulations or can degrade during harsh production processes, such as extrusion or pelleting.' 

To minimize such risks and protect the power of astaxanthin, AstaReal uses encapsulation in its animal nutrition brand NOVASTA®. Its recently launched NOVASTA® EB15 can be mixed into pet food or added to supplements and consists of algal flour (32%) encapsulated in rapeseed oil with a final astaxanthin concentration of 1.5%. Due to encapsulation, it can be better incorporated into challenging formulations, such as pellets, meal mixes, and soft chews, which are more likely to be exposed to air at ambient temperature.

If manufacturers are sensitive to the shifting demands of pet parents and are willing to combine health with sustainability trends, they are well set. Algal astaxanthin can play an assisting role in these efforts. Thanks to its antioxidant and multifunctional effects, it naturally supports the well-being of our four-legged friends while stemming from a futureproof source.
Futureproof Feeding
One of today's major challenges is how to feed the planet sustainably. In search of plant-based sources for both human and animals, algae are gaining attention. Algae are relatively easy to cultivate, are nutritious, and being considered as more sustainable than several conventional crops. One precious and health-promoting ingredient that algae offer is astaxanthin. AstaReal derives the carotenoid from the micoralgae Haematococcus pluvialis that are being cultivated indoor in specially designed photobioreactors. The company applies a unique system to reuse the excess heat produced during the algae cultivation process to heat up 2500 apartments in the nearby residential area, aiming to continuously reduce their carbon footprint.   By AstaReal
Source: All Pet Food Magazine
References
1.    Mordor Intelligence. 'Europe Pet Food Market SIZE & SHARE ANALYSIS - GROWTH TRENDS & FORECASTS UP TO 2029.' https://www.mordorintelligence.com/industry-reports/pet-food-market-in-europe-industry. Accessed Feb. 23, 2024.
2.    B.M. Zanghi, et al., 'Effects of Postexercise Feeding of a Supplemental Carbohydrate and Protein Bar with or without Astaxanthin from Haematococcus pluvialis to Exercise-Conditioned Dogs,' Am. J. Vet. Res. 76(4), 338–350 (2015).
3.    T. Murai, et al., 'Effects of Astaxanthin Supplementation in Healthy and Obese Dogs,' Veterinary Medicine: Research and Reports 10, 29–35 (2019).
4.    National Center for Biotechnology Information. 'PubChem Patent Summary for US-9820497-B2, Astaxanthin-containing pet foods.' https://pubchem.ncbi.nlm.nih.gov/patent/US-9820497-B2. Accessed Feb. 23, 2024.
5.    J.S. Park, et al., 'Astaxanthin Modulates Age-Associated Mitochondrial Dysfunction in Healthy Dogs,' Journal of Animal Science 91(1), 268–275 (2013).
6.    B.P. Chew, et al., 'Dietary Astaxanthin Enhances Immune Response in Dogs,' Veterinary Immunology and Immunopathology 140(3–4), 199–206 (2011).
7.    J.S. Park, et al., 'Astaxanthin Stimulates Cell-Mediated and Humoral Immune Responses in Cats,' Veterinary Immunology and Immunopathology 144, 455–461 (2011).
8.    W. Wang, et al., 'Antioxidant Supplementation Increases Retinal Responses and Decreases Refractive Error Changes in Dogs,' Journal of Nutritional Science 5, E18 (2016).


Proteins

Proteins Hydrolyzed Proteins: What They Are and How They Act in the Management of Food Sensitivities and Chronic Enteropathies

6+ MIN

Hydrolyzed Proteins: What They Are and How They Act in the Management of Food Sensitivities and Chronic Enteropathies

From Ingredient Selection to Molecular Bioavailability
In recent years, the quality standards of foods intended for dogs and cats have undergone changes and redefinitions. While for decades, one of the most attractive aspects of commercial diets was the use of premium meats or exotic protein sources, the current focus has shifted toward the nutrients present in each ingredient and their bioavailability within the diet. 
  Advances in animal nutrition studies have encouraged a more precise and effective approach, based on the principle that animals do not require specific ingredients, but rather essential nutrients in adequate amounts. This redefinition in ingredient selection represents a movement toward maximizing nutritional efficiency, with a focus on greater nutrient bioavailability, contributing to the health and well-being of companion animals.
Why Protein Size Matters
The molecular structure of food is important because the immune system interacts with dietary proteins through the recognition of epitopes—mechanisms that are still not fully understood—formed by specific amino acid sequences present on the surface of large polypeptides. In sensitized animals, these proteins may bind to immunoglobulin E present on the surface of mast cells, triggering a cascade of inflammatory mediators that results in the clinical signs of adverse food reactions, such as intense pruritus and gastrointestinal alterations.

For a long time, the strategy used to address this condition was the search for different proteins, derived from novel sources to which animals had not yet been exposed and, therefore, would have a lower probability of previous sensitization. However, ensuring this 'novelty' and the constant risk of cross-contamination make this approach increasingly difficult and less reliable, being effective only in some cases.

Hydrolysis: The 'Molecular Disguise' of Proteins
Advances in animal nutrition have prioritized the so-called 'molecular disguise' of conventional proteins through partial or extensive enzymatic hydrolysis, which essentially is the structural modification of proteins through hydrolysis.

The hydrolysis process promotes the breakdown of intact proteins, generally with molecular weights between 15 and 70 kDa, into small peptides and free amino acids, reducing their molecular weight to values below the threshold of immune recognition. In this way, the antigenic 'target' of immunoglobulins is minimized without significantly altering the nutritional value of food.

For a protein source to be considered hydrolyzed and effective in the management of food allergies, most of its peptides should have a molecular mass below 10 kDa, with the presence of much smaller protein fractions (below 500 Da) being common. Recent characterizations illustrate the precision and efficiency of modern processes, demonstrating that several protein ingredients—from both animal and plant origins—can achieve molecular profiles with a low probability of detection by the immune system.
Hypoallergenic and Anallergenic Diets
This has opened the opportunity for the development of hypoallergenic diets, characterized by being composed of few ingredients and often by a single protein source subjected to partial or total hydrolysis before being included in the formulation. These diets are characterized by adequate digestibility, reduced allergenic potential, and a good amino acid balance, with high bioavailability of essential amino acids.

In addition, there are purified diets, also known as anallergenic diets, in which the protein is supplied with an extremely high degree of hydrolysis, resulting in very small peptides that are practically undetectable by the immune system. Hydrolyzed diets, therefore, offer advantages for patients with deficiencies in protein digestion mechanisms or for those affected by enteropathies responsive to intact dietary proteins.
Chronic Enteropathies and Nutritional Support
Patients with chronic enteropathies present a group of gastrointestinal conditions characterized by persistent or recurrent signs, such as diarrhea, vomiting, weight loss, changes in fecal quality, and reduced intestinal absorptive capacity. In these cases, the intestinal mucosa may present prolonged inflammation, alterations in the intestinal barrier, dysbiosis, and compromised villi, which are essential structures for adequate nutrient utilization. These patients often represent a major challenge for their owners and the veterinary team, and their quality of life may be significantly compromised when effective therapeutic strategies are not established.

The advent of diets containing hydrolyzed or extensively hydrolyzed proteins represents a milestone in companion animal nutrition. These formulations are fundamental for the recovery and quality of life of patients affected by inflammatory bowel disease or other digestive disorders related to dietary proteins, as they allow the restoration of digestive function, adequate amino acid absorption, and tissue recovery, favoring weight gain, maintenance of muscle mass, and remission of clinical signs.
Clinical Applications and Perspectives
In dogs with food-responsive diarrhea, hydrolyzed diets frequently promote rapid resolution of clinical signs as they are both diagnostic and therapeutic tools in cases of food allergy. In dogs with more complex conditions, such as inflammatory bowel disease, the use of hydrolyzed proteins is essential to minimize the development of sensitization to new dietary antigens.

The supply of 'pre-digested' peptides helps reduce the antigenic load of the diet while maintaining the animal's nutritional support, and providing the energy required for tissue repair processes. Recent studies have also explored the use of hydrolyzed proteins derived from poultry liver, feathers, viscera, fish, beef, and insects to improve fecal quality and modulate intestinal fermentation in dogs, highlighting the versatility of these protein sources in clinical settings.
Conclusion
The transition from an ingredient-focused approach to one centered on molecular bioavailability defines modern companion animal nutrition. As our understanding of the pathophysiology of disorders affecting dogs and cats deepens, the selection of raw materials will continue to be evaluated based on their effective contribution to metabolic health.

This shift is not limited to the prevention of food allergies but seeks to optimize nutrient utilization by animals. By prioritizing adequate molecular weight, high digestibility, and modulation of the intestinal environment, the industry develops solutions that translate directly into improved quality of life for patients. The true indicator of quality lies in the ability to provide nutrients in a bioavailable, safe, and consistent way, allowing the survival of patients who previously had limitations almost incompatible with life. By Paula K. Velho¹, Gustavo de A. Fusquine¹, Laura S. de Freitas¹, Eduarda B. F. de Oliveira¹, Luciano Trevizan¹
Source: All Pet Food Magazine

¹GENAC – Grupo de Estudos em Nutrição de Animais de Companhia da Universidade Federal do Rio Grande do Sul
  References
FASCETTI, Andrea J. et al. (Ed.). Applied veterinary clinical nutrition. 2012.
MARTELLO, Elisa et al. Liquid Hydrolyzed Fish Protein (Anchovy) in the Canine Diet: A Focus on Gut Fermentation and Fecal Quality. Veterinary Sciences, v. 12, n. 8, p. 779, 2025. DOI: 10.3390/ vetsci12080779.
MARTÍNEZ-LÓPEZ, Lina María et al. Effect of sequentially fed high protein, hydrolyzed protein, and high fiber diets on the fecal microbiota of healthy dogs: a cross-over study. Animal microbiome, v. 3, n. 1, p. 42, 2021. DOI: 10.1186/s42523-021-00101-8.
PINTO, Caroline Fredrich Dourado et al. Hydrolyzed chicken liver used as single source of animal protein in diet and its effect on cytokines, immunoglobulins, and fecal microbiota profile of adult dogs. PLoS One, v. 17, n. 7, sk, 2022. DOI: 10.1371/journal.pone.0271932.
PINTO, Caroline Fredrich Dourado et al. Characterisation of spray dried hydrolysed chicken liver powder: effects on palatability and digestibility when included as single source of animal protein in dog diets. Italian Journal of Animal Science, v. 20, n. 1, p. 2086-2094, 2021. DOI: 10.1080/1828051X.2021.1993091.
PINTO, Caroline FD et al. Effects of hydrolyzed chicken liver on digestibility, fecal and urinary characteristics, and fecal metabolites of adult dogs. Journal of Animal Science, v. 101, p. 1-12, 2023. DOI: 10.1093/jas/skad366.
PINTO, Caroline Fredrich Dourado et al. Effects of diets based on hydrolyzed chicken liver and different protein concentrations on the formation and deamination of biogenic amines and total antioxidant capacity of dogs. Animals, v. 13, n. 16, p. 2578, 2023. DOI: 10.3390/ani13162578
SEZEROTTO, Pamela Prestes et al. Hydrolyzed chicken liver and increasing crude protein levels on palatability, digestibility, and intestinal fermentation products of cats. Journal of Animal Science, v. 103, skaf173, 2025. DOI: 10.1093/jas/skaf173.
 

Proteins Innovative Functional Protein Solutions for the Next Generation of Dry and Wet Pet Food

4+ MIN

Innovative Functional Protein Solutions for the Next Generation of Dry and Wet Pet Food

In addition to being a high-quality protein source, plasma provides highly relevant technological properties for industrial processing. In wet pet food applications, for example, it acts as an excellent binder, emulsifier, and gelling agent, directly contributing to texture, stability, and final product appearance. The pet food industry has used plasma in wet formulations for more than 30 years because of its ability to improve water retention, increase chunk consistency, create improved texture, richer gravies, and provide greater juiciness and cohesion to the food. 

The studies demonstrate that plasma inclusion significantly improves hardness, elasticity, chewiness, and structural integrity in wet pet food chunks. In fish and salmon applications, for example, a linear increase in hardness and consistency was observed as plasma inclusion levels increased, along with better gravy absorption and greater formulation stability. Compared to traditional ingredients such as wheat gluten, egg albumin, carrageenan, and soy protein concentrate, plasma showed an excellent balance between gel strength, emulsification, and water retention. 

Another important aspect is that technological benefits go hand in hand with nutritional improvements. Digestibility studies in cats demonstrated significant improvements in protein, fat, mineral, and energy digestion in diets containing plasma, along with increased palatability. In comparative trials, foods containing plasma were preferred over formulations using wheat gluten and other protein sources, reinforcing plasma's contribution to feed acceptance and the sensory experience for pets. 

In dry pet food, plasma also delivers significant results. Its application in kibble, treats, toppers, snacks, and supplements improves both processing quality and the functional benefits of the final product. During extrusion, plasma contributes to greater kibble durability, reduced fines, improved physical structure, and greater stability in formulas containing high levels of fresh meat. The studies showed that inclusion levels between 2.5% and 5% allowed manufacturers to formulate with higher meat content while maintaining excellent kibble durability and starch gelatinization. 

In addition, plasma offers high solubility and easy incorporation into different formulations, allowing partial replacement of ingredients such as gluten, egg albumin, and gums, providing both economic and technological advantages. In dry dog food, studies also demonstrated improvements in dry matter and protein digestibility, along with a 10–20% reduction in fecal output, indicating better nutrient utilization and improved stool quality. 

Palatability is another major highlight. In comparative studies against spray-dried liver, plasma-containing diets showed a higher consumption ratio in cats when included in extruded formulations. These results indicate that plasma can serve as a highly palatable functional alternative to enhance palatability which may allow alterations in the use of traditional palatants. 

However, plasma benefits extend beyond nutrition and processing. The ingredient contains bioactive functional proteins capable of positively modulating immune and inflammatory responses. Several studies have demonstrated that plasma helps maintain intestinal integrity, reduces mucosal permeability, and contributes to immune balance. Reductions in pro-inflammatory cytokines, such as TNF-α, along with increases in IL-10, an important anti-inflammatory cytokine, were observed. These findings indicate improved intestinal barrier function and systemic digestive health support. 

Within this context, new studies related to the intestinal microbiome further reinforce plasma's position as a high-value functional ingredient. In adult dogs fed with extruded diets containing plasma, researchers observed a linear increase in short-chain fatty acid (SCFA) production, important markers of beneficial fermentation and positive intestinal microbiota activity. Increases in beneficial bacteria, such as Lactobacillus, Streptococcus, and Catenibacterium, were also observed, along with reductions in metabolites associated with undesirable protein fermentation, indicating a healthier intestinal environment. 

Another relevant finding was the increase in fecal IgA, suggesting improved intestinal immune homeostasis and stronger mucosal immunity. The study concluded that plasma promotes consistent, inclusion-dependent positive responses, contributing to intestinal health, microbiome modulation, and improved protein metabolism efficiency. 

Recent studies have also expanded the potential application of plasma for joint health and mobility formulations targeting senior dogs. In dogs with osteoarthritis fed diets containing 4% plasma, significant improvements in mobility and reductions in lameness scores were observed after 42 days. In addition, plasma reduced inflammatory markers such as IL-1β and increased IL-10, demonstrating a positive effect on the inflammatory response associated with osteoarthritis. 

Supplemented animals also demonstrated greater antioxidant capacity, evidenced by increased SOD (superoxide dismutase) activity and reduced MDA (malondialdehyde) levels, as well as lower levels of MMP-2 and MMP-13, enzymes associated with articular cartilage degradation. At the same time, protein digestibility improved without negatively affecting feed intake or fecal scores. These results demonstrate that plasma can serve as a strategic functional ingredient in senior dog diets, contributing to mobility, joint health, inflammatory control, and antioxidant support. 

Another important ingredient is the spray-dried red blood cells in pet food. Spray-dried red blood cells emerge as a functional solution that combines natural coloration and highly digestible protein in a single ingredient. Their main application is related to creating a natural meat-like color in dry and wet pet foods, helping reduce or eliminate artificial colorants while delivering a more natural and premium appearance to formulations. 

In dry pet food, color intensity progressively increases according to inclusion level, providing kibble with a more meat-like appearance. In pâté-like wet pet food, red blood cells intensify the natural meat appearance and enhance the visual authenticity of the final product. Beyond visual appeal, the ingredient also provides highly digestible protein and an excellent amino acid profile, including high levels of lysine, leucine, and valine. 

Overall, both spray-dried plasma and spray-dried red blood cells represent a new generation of functional ingredients for pet food, capable of combining processing technology, nutritional value, intestinal health, systemic functionality, and premium appeal into a single component. The studies presented reinforce that these ingredients offer complete solutions for the industry, meeting current demands for more functional, natural, efficient formulations aligned with trends in animal health and wellness. By Dr. Joy Campbell, Senior Director of Global Pet Food Technical Services, APC
Source: All Pet Food Magazine


Minerals

Minerals The Most Underrated Tool In Pet Nutrition

2+ MIN

The Most Underrated Tool In Pet Nutrition

Trace minerals are involved in most health outcomes nutritionists aim to influence and pet parents care deeply about: immune resilience, skin and coat health, antioxidant defense, mobility, cellular function, vitality and whole-body health.

They're not acting in isolation. They work behind the scenes, supporting the biological systems that help every other nutritional strategy perform effectively.

For today's pet food brands, every ingredient must justify its place in the diet — not only the high-inclusion ingredients, the ones with easy consumer stories or the ones that look innovative on paper. Every ingredient. That is where I see an opportunity.

Innovation is not always what comes next, it is expecting more from what is already there.

Because trace minerals are foundational, they can become familiar and often treated as 'already solved', receiving less strategic evaluation than newer or more visible ingredients.

Mineral programs can remain unchanged for years. Familiarity creates comfort. Comfort creates confidence.

Yet, source, structure and delivery influence how effectively a trace mineral supports animal health. When those components are optimized, a mineral moves from simply meeting a requirement to supporting broader formula performance and pets' wellbeing. 

That distinction matters. 

At Zinpro Corporation, we've spent more than five decades studying trace minerals across species, generating research behind our performance minerals. One lesson continues to stand out: biology is remarkably consistent. Cells still require nutrients to function. Tissues still need support to repair and respond. 

In livestock, performance is evaluated through productivity, efficiency, disease resistance, and profitability. In pets, focus shifts to quality of life, longevity, overall well-being and human-animal bond. Outcomes measured may look different, but the underlying mechanisms don't change. 

It is time to start expecting more from what is already there and stop leaving opportunity on the table — for brands, for formulas and for the pets counting on us.  By Allison Millican, PhD
Source: Zinpro Corporation

Functional Additives Sinergia and the Role of Probiotics in Supporting Digestive Health in Pets

5+ MIN

Sinergia and the Role of Probiotics in Supporting Digestive Health in Pets

After years of focusing solely on basic requirements, advancements in microbiology and functional nutrition have positioned intestinal health as a key pillar for animal well-being. Microbiota research has evolved beyond basic science and is now driving the development of value-added pet foods.

Today, pet food manufacturers, veterinarians, and pet owners recognize that the balance in the intestinal microbiota directly impacts digestion, nutrient use, immune response, and pets' quality of life. Thus, there is a transformation in food formulation and the development of functional ingredients.  
Why is Microbiota Now a Key Factor in Pet Food?
The intestinal microbiota is composed of billions of microorganisms that interact continuously with the host. Far away from having a passive role, they participate in crucial digestive, metabolic, and immune functions.

A balanced microbiota is now known for its contributions to the integrity of the intestinal barrier, improved nutrient utilization, and the maintenance of physiological mechanisms associated with overall health. In other words, alterations in the microbiota's composition can negatively impact pets' well-being and digestive function.
What is the Role of Probiotics Within This Nutritional Approach?
Probiotics are among the most relevant tools for supporting microbiota balance. Their function goes beyond incorporating live microorganisms into food: they create favorable conditions for the intestinal ecosystem and contribute to overall animal well-being.

Current research focuses on understanding which microorganisms are incorporated, the metabolites they produce, and their interactions with the resident microbiota. This different approach is becoming a key component in modern functional nutrition.

For manufacturers, this shift represents a concrete opportunity to develop science-based foods with differentiated characteristics that align with the increasing demand for products oriented toward digestive well-being and integral health.
What Characteristics Must a Probiotic Have to Provide Real Benefits in Pets?
 
Not all probiotics are equally functional. Efficacy depends on many factors, including strain selection, stability during production, and the ability to reach the gastrointestinal tract.
  In pet food, the primary technological challenge is ensuring microbial viability under severe conditions during extrusion and drying. This is why spore-forming microorganisms represent a major technological breakthrough. Endospores act as a natural protector against high temperatures, mechanical stress (pressure and shearing), and long periods of storage (shelf-life)—ensuring that the Colony-Forming Units (CFU) declared on the packaging remain viable until they reach the pet's bowl.    'Sinergia by Adinnova is a solution developed to support the new generation of functional food oriented to the digestive well-being of dogs and cats'   In addition, strain selection must consider individual benefits, metabolite generation, and the interactions within the intestinal ecosystem.   How Was Sinergia Developed and What Market Needs Does It Address?
At Adinnova, we noticed a clear evolution in the pet food market's needs. Manufacturers look for solutions that differentiate their products through real functional benefits, supported by scientific evidence and compatible with modern industrial processes.

From our experience in applied microbiology and the development of biotechnological solutions for animal nutrition, we identified the need to address intestinal health from a broad perspective, considering not only microorganisms but also functional metabolites that participate in microbiota balance.

Sinergia by Adinnova was created under this vision—it is a solution designed to support the new generation of functional foods oriented toward the digestive well-being of dogs and cats.
Why is Sinergia Different from Other Available Solutions?
The main characteristic of Sinergia is its symbiotic ecosystem design. It is not formulated simply by different microorganisms but also a strategic consortium selected by its complementary mechanisms of action:
  Bacillus consortium (thermostability and digestibility): It is composed of Bacillus subtilis, Bacillus licheniformis, and Bacillus coagulans. In addition to their resistance to extrusion, these strains act as bio-factories in the intestine, producing extracellular enzymes (protease, amylase, and lipase) that maximize nutrient utilization. B. coagulans also contributes to lactic acid production, which is fundamental to the modulation of intestinal pH and competitive control of pathogens.   'More than an additive, Sinergia by Adinnova is a driver of functional metabolites designed to impact systemic health from the gastrointestinal tract'   Clostridium butyricum (intestinal barrier integrity): It stands out for being the primary producer of butyric acid. This short-chain fatty acid is the main source of energy for colonocytes; thus, it is essential for maintaining the epithelia's tight junctions, preventing leaky gut syndrome, and modulating local inflammatory responses.
  Saccharomyces cerevisiae (probiotic immune modulation): As a live, biologically active probiotic yeast, it provides a differentiated cellular approach compared to bacteria in the formula. It promotes the digestive ecosystem's balance and stability, while its components within the cell wall—rich in glucans and mannooligosaccharides (MOS)—actively intercept pathogens by stimulating the Gut-Associated Lymphoid Tissue.
  More than an additive, Sinergia by Adinnova is a driver of functional metabolites designed to impact systemic health from the gastrointestinal tract.   What Benefits Does Sinergia Offer to Food Manufacturers and to the Pets That Consume Those Products?

For manufacturers, Sinergia is an innovative tool grounded in applied microbiology, enabling the development of valuable solutions aligned with global trends in functional nutrition and intestinal health.
  On the other hand, the goal is to support the balance of the microbiota in dogs and cats through microorganisms and functional metabolites that help maintain favorable conditions for digestive health and welfare.   The Future of Functional Nutrition  
A deeper understanding of the gut microbiota is shaping the future of pet nutrition. Nutritional solutions are evolving from conventional formulations toward comprehensive strategies that harness the interactions among microorganisms, functional metabolites, and the host.
  At Adinnova, we believe that the future of pet food will increasingly depend on a deep understanding of microbial ecosystems and the ability to translate knowledge into innovative solutions for the industry. By Gonzalo Vicente MEng, R&D Director at Adinnova
Source: All Pet Food Magazine

About Adinnova
Adinnova is an Argentine company, present in international markets, dedicated to the development of natural additives for animal nutrition. Each bio-solution integrates science and innovation applied to wellbeing and productivity.
More information on our website: adinnova.com.ar

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